Cytotoxic effects of butanolic extract from Pfaffia paniculata (Brazilian Ginseng) on cultured human breast cancer cell line MCF-7

Autor(es):Mostrar menos -

Nagamine, Marcia Kazumi ^[1] ; da Silva, Tereza Cristina ^[1] ; Matsuzaki, Patricia ^[1] ; Pinello, Katia Cristina ^[1] ; Cogliati, Bruno ^[1] ; Pizzo, Celia Regina ^[2] ; Akisue, Gokithi ^[3] ; Haraguchi, Mitsue ^[4] ; Gorniak, Silvana Lima ^[1] ; Sinhorini, Idercio Luiz ^[1] ; Kesava Rao, Kurapati Venkata ^[1] ; Marzagao Barbuto, Jose Alexandre ^[2] ; Zaidan Dagli, Maria Lucia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Pathol, Fac Vet Med & Zootechny, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Immunol, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil

^[3] Univ Sao Francisco, Fac Pharmaceut Sci, BR-12900000 Braganca Paulista, SP - Brazil

^[4] Biol Inst Sao Paulo, Pharmacol Sect, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY; v. 61, n. 1, p. 75-82, JAN 2009.
• Citações Web of Science: 22

Resumo

Roots of Pfaffia paniculata have been well documented for multifarious therapeutic values and have also been used for cancer therapy in folk medicine. This study has been performed in a human breast tumor cell line, the MCF-7 cells. These are the most commonly used model of estrogen-positive breast cancer, and it has been originally established in 1973 at the Michigan Cancer Foundation from a pleural effusion taken from a woman with metastatic breast cancer. Butanolic extract of the roots of P. paniculata showed cytotoxic effect MCF-7 cell line. as determined with crystal violet assay, cellular death with acridine orange/ethidium bromide staining, and cell proliferation with immunocytochemistry of bromodeoxyuridine (BrdU). Subcellular alterations were evaluated by electron microscopy. Cells treated With butanolic extract showed degeneration of cytoplasmic components and profound morphological and nuclear alterations. The results show that this butanolic extract indeed presents cytotoxic substances, and its fractions merit further investigations. (C) 2008 Elsevier GmbH. All rights reserved. (AU)

• Processo FAPESP: 01/06820-2 - Carcinogenese pulmonar em camundongos portadores de delecao em um dos alelos da conexina 43 (cx43+/-).
• Beneficiário: Maria Lucia Zaidan Dagli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 02/05083-7 - Efeitos da Pfaffia paniculata sobre a proliferação celular e a capacidade de comunicação intercelular por junções comunicantes do tipo gap em linhagens tumorais de células mamárias humanas
• Beneficiário: Márcia Kazumi Nagamine
• Modalidade de apoio: Bolsas no Brasil - Mestrado