Comparison of Cross-Sectional Hardness and Transverse Microradiography of Artificial Carious Enamel Lesions Induced by Different Demineralising Solutions and Gels

Autor(es)

Magalhaes, A. C. ^[1] ; Moron, B. M. ^[1] ; Comar, L. P. ^[1] ; Wiegand, A. ^[2] ; Buchalla, W. ^[2] ; Buzalaf, M. A. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Biol Sci, Bauru Sch Dent, BR-17012901 Bauru, SP - Brazil

^[2] Univ Zurich, Clin Prevent Dent Periodontol & Cariol, Zurich - Switzerland

• Tipo de documento: Artigo Científico
• Fonte: Caries Research; v. 43, n. 6, p. 474-483, 2009.
• Citações Web of Science: 43

Resumo

The aims of this study were: (1) to correlate surface (SH) and cross-sectional hardness (CSH) with microradiographic parameters of artificial enamel lesions; (2) to compare lesions prepared by different protocols. Fifty bovine enamel specimens were allocated by stratified randomisation according to their initial SH values to five groups and lesions produced by different methods: MC gel (methylcellulose gel/lactic acid, pH 4.6, 14 days); PA gel (polyacrylic acid/lactic acid/hydroxyapatite, pH 4.8, 16 h); MHDP (undersaturated lactate buffer/methyl diphosphonate, pH 5.0, 6 days); buffer (undersaturated acetate buffer/fluoride, pH 5.0, 16 h), and pH cycling (7 days). SH of the lesions (SH(1)) was measured. The specimens were longitudinally sectioned and transverse microradiography (TMR) and CSH measured at 10- to 220-mu m depth from the surface. Overall, there was a medium correlation but non-linear and variable relationship between mineral content and root CSH. root SH(1) was weakly to moderately correlated with surface layer properties, weakly correlated with lesion depth but uncorrelated with integrated mineral loss. MHDP lesions showed the highest subsurface mineral loss, followed by pH cycling, buffer, PA gel and MC gel lesions. The conclusions were: (1) CSH, as an alternative to TMR, does not estimate mineral content very accurately, but gives information about mechanical properties of lesions; (2) SH should not be used to analyse lesions; (3) artificial caries lesions produced by the protocols differ, especially considering the method of analysis. Copyright (C) 2009 S. Karger AG, Basel (AU)

• Processo FAPESP: 08/01472-5 - Avaliação da efetividade de diferentes métodos para produção e análise de lesão de cárie artificial in vitro
• Beneficiário: Livia Picchi Comar
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 09/03581-9 - Comparação entre diferentes métodos para produção e análise de lesão de cárie artificial em esmalte e dentina
• Beneficiário: Bruna Mangialardo Moron
• Modalidade de apoio: Bolsas no Brasil - Mestrado