A Simple Method for the Quantitative Analysis of Tyrosol by HPLC in Liquid Czapek Cultures from Endophytic Fungi

Autor(es)

Guimaraes, Denise O. ^[1] ; Borges, Keyller B. ^[2] ; Bonato, Pierina S. ^[2] ; Pupo, Monica T. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Ciencias Farmaceut, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Quim & Fis, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 20, n. 1, p. 188-194, 2009.
• Citações Web of Science: 6

Resumo

Tyrosol is a possible quorum sensing molecule in endophytic fungi. High-performance liquid chromatography (HPLC) coupled with diode array detector (DAD) was used for the analysis of tyrosol in liquid Czapek fungal cultures. The optimized conditions were gradient mobile phase, in linear mode, consisting initially of acetonitrile/water (1:9 v/v) and increasing up to acetonitrile (100%) in 30 minutes at a flow rate of 1 mL min(-1). The column used was a ZORBAX (R) ODS (250 x 4.6 mm, 5 mu m) at 25 degrees C. Liquid-liquid extraction of 0.5 mL medium (pH 7.0) with ethyl acetate and injection of 20 mu L after solvent evaporation under air flow gave good results. Some validation parameters obtained were: linearity 0.0125-5.0 mu g mL(-1) medium (r = 0.9967), quantification limit of 0.0125 mu g mL(-1) medium, %CV (precision) and %E (accuracy) bellow 15% and recovery around 80%. Therefore, the developed method presented satisfactory validation parameters and it was efficient for the analysis of tyrosol in Czapek medium. (AU)

• Processo FAPESP: 03/07535-5 - Fungos endofíticos associados a espécies da Asteraceae: isolamento, cultivo, identificação de metabólitos secundários e atividade biológica
• Beneficiário: Mônica Tallarico Pupo
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 04/07935-6 - Bioprospecção de fungos: a busca de compostos importantes para o projeto de remédios e enzimas para aplicações farmacêuticas e industriais
• Beneficiário: Jairo Kenupp Bastos
• Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 05/03791-2 - Estudos químicos e biológicos sobre a produção de metabólitos em dois fungos endofíticos (va1 e va15) associados a Viguiera arenaria
• Beneficiário: Denise Oliveira Guimarães
• Linha de fomento: Bolsas no Brasil - Doutorado