A simple method for the quantitative analysis of tyrosol by hplc in liquid Czapek Cultures from endophytic fungi

Autor(es)

Guimaraes, Denise O. ^[1] ; Borges, Keyller B. ^[2] ; Bonato, Pierina S. ^[2] ; Pupo, Monica T. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Ciencias Farmaceut, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Quim & Fis, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 20, n. 1, p. 188-194, 2009.
• Citações Web of Science: 6

Resumo

Tyrosol is a possible quorum sensing molecule in endophytic fungi. High-performance liquid chromatography (HPLC) coupled with diode array detector (DAD) was used for the analysis of tyrosol in liquid Czapek fungal cultures. The optimized conditions were gradient mobile phase, in linear mode, consisting initially of acetonitrile/water (1:9 v/v) and increasing up to acetonitrile (100%) in 30 minutes at a flow rate of 1 mL min-1. The column used was a ZORBAX® ODS (250 × 4.6 mm, 5 μm) at 25 ºC. Liquid-liquid extraction of 0.5 mL medium (pH 7.0) with ethyl acetate and injection of 20 μL after solvent evaporation under air flow gave good results. Some validation parameters obtained were: linearity 0.0125-5.0 μg mL-1 medium (r = 0.9967), quantification limit of 0.0125 μg mL-1 medium, %CV (precision) and %E (accuracy) bellow 15% and recovery around 80%. Therefore, the developed method presented satisfactory validation parameters and it was efficient for the analysis of tyrosol in Czapek medium. (AU)

• Processo FAPESP: 03/07535-5 - Fungos endofiticos associados a especies da asteraceae: isolamento, cultivo, identificacao de metabolitos secundarios e atividade biologica.
• Beneficiário: Mônica Tallarico Pupo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/03791-2 - Estudos quimicos e biologicos sobre a producao de metabolitos em dois fungos endofiticos (va1 e va15) associados a viguiera arenaria.
• Beneficiário: Denise Oliveira Guimarães
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 04/07935-6 - Bioprospecção de fungos: a busca de compostos importantes para o projeto de remédios e enzimas para aplicações farmacêuticas e industriais
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático