Homogenous amniotic membrane as a biological dressing for oral mucositis in rats: Histomorphometric analysis

Autor(es)

Vilela-Goulart, Maria das Gracas ^[1] ; Santos Teixeira, Raquel Teodoro ^[1] ; Ranyel, Daniela Coelho ^[2] ; Niccoli-Filho, Walter ; Gomes, Monica Fernandes ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Assoc Prosaude Pacientes Necessidades Especiais A, Special Hlth Care Needs Assoc, Sao Jose Dos Campos, SP - Brazil

^[2] Sao Paulo State Univ, UNESP, Sao Jose Campos Dent Sch, Dept Diag & Surg, Sao Jose Dos Campos, SP - Brazil

^[3] Sao Paulo State Univ, UNESP, Ctr Biociencias Aplicado Pacientes Necessid, Sao Jose Campos Dent Sch, CEBAPE, Dept Biosci & Oral Diag, Biosci Ctr Special Hlth C, Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 53, n. 12, p. 1163-1171, DEC 2008.
• Citações Web of Science: 12

Resumo

Objective: oral mucositis is a frequent and significant side effect of chemotherapy and/or radiation therapy. The purpose of this study was to evaluate the effects of the homogenous amniotic membrane (HAM) as a biological dressing for oral mucositis induced by 5-fluorouracil (5-FU) in the labial fornix region of inferior incisors in rats. Methods: Sixty rats were divided into three groups: control (50% acetic acid), 5-FU (50% acetic acid and 5-fluorouracil-induced oral mucositis) and 5-FU + HAM (50% acetic acid and 5fluorouracil-induced oral mucositis, and treated with HAM). Histological analyses were done 3, 7, 14 and 21 days after applying the acetic acid. Results: HAM showed not only no signs of rejection as well as an excellent tissue adherence to the ulcerated surface. Histomorphometric analysis indicated a lower quantity of inflammatory cells in the 5-FU + HAM group in all periods when compared to the other groups and there was a statistically significant difference in the 3- and 7-day periods (P < 0.001). Conclusions: The results of this study suggest that HAM was biocompatible with great adhesive power to the ulcerated surface, accelerated the healing process and exhibited anti-inflammatory activity. Further studies should be done to investigate the potential benefits of this material in clinical trials. HAM could lead to improved results as a biological dressing in treatment and management of oral mucositis in patients submitted to chemotherapy and/or radiation therapy. (C) 2008 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 04/04447-0 - Efeitos do extrato proteico da membrana amniotica homogena como curativo em mucosite oral induzida pela 5-fluorouracil: analise histomorfometrica e avaliacao dos parametros bioquimicos de saliva.
• Beneficiário: Daniela Coelho Rangel
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica