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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Melatonin and the circadian entrainment of metabolic and hormonal activities in primary isolated adipocytes

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Autor(es):
Cardoso Alonso-Vale, Maria Isabel [1] ; Andreotti, Sandra [1] ; Mukai, Paula Yuri [1] ; Borges-Silva, Cristina das Neves [1] ; Peres, Sidney Barnabe [1] ; Cipolla-Neto, Jose [1] ; Lima, Fabio Bessa [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508900 Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Journal of Pineal Research; v. 45, n. 4, p. 422-429, NOV 2008.
Citações Web of Science: 60
Resumo

The aim of this work was to investigate the effect of the in vitro circadian-like exposure to melatonin {[}in the presence or absence of insulin (Ins)] on the metabolism and clock gene expression in adipocytes. To simulate the cyclic characteristics of the daily melatonin profile, isolated rat adipocytes were exposed in a circadian-like pattern to melatonin added to the incubating medium for 12 hr (mimicking the night), followed by an equal period without melatonin (mimicking the day) combined or not with Ins. This intermittent incubation was interrupted when four and a half 24-hr cycles were fulfilled. At the end, either during the induced night (melatonin present) or the induced day (melatonin absent), the rates of lipolysis and D-{[}U-(14)C]-glucose incorporation into lipids were estimated, in addition to the determination of lipogenic {[}glucose-6-phosphate dehydrogenase and fatty acid synthase (FAS)] and lipolytic (hormone sensitive lipase) enzymes and clock gene (Bmal-1b, Clock, Per-1 and Cry-1) mRNA expression. The leptin release was also measured. During the induced night, the following effects were observed: an increase in the mRNA expression of Clock, Per-1 and FAS; a rise in lipogenic response and leptin secretion; and a decrease in the lipolytic activity. The intermittent exposure of adipocytes to melatonin temporally and rhythmically synchronized their metabolic and hormonal function in a circadian fashion, mimicking what is observed in vivo in animals during the daily light-dark cycle. Therefore, this work helps to clarify the physiological relevance of the circadian pattern of melatonin secretion and its interactions with Ins, contributing to a better understanding of the adipocyte biology. (AU)

Processo FAPESP: 04/06767-2 - O papel da melatonina no controle do metabolismo energético: interações hormonais, ações centrais e periféricas (pineal, obesidade, diabetes e envelhecimento)
Beneficiário:José Cipolla Neto
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 04/14696-8 - Regulacao pela melatonina da expressao genica e das funcoes endocrinas e metabolicas de adipocitos isolados de tecido adiposo branco em cultura primaria.
Beneficiário:Maria Isabel Cardoso Alonso-Vale
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado