| Texto completo | |
| Autor(es): |
Fontana, Karina
[1, 2]
;
Aldrovani, Marcela
[3]
;
de Paoli, Flavia
[3]
;
Oliveira, Helena C. F.
[4]
;
Vidal, Benedicto de Campos
[3]
;
da Cruz-Hoefling, Maria Alice
[2]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] State Univ Campinas UNICAMP, Fac Med Sci, Dept Pharmacol, BR-13083970 Campinas, SP - Brazil
[2] State Univ Campinas UNICAMP, Inst Biol, Dept Histol & Embryol, BR-13083970 Campinas, SP - Brazil
[3] State Univ Campinas UNICAMP, Inst Biol, Dept Cell Biol, BR-13083970 Campinas, SP - Brazil
[4] State Univ Campinas UNICAMP, Inst Biol, Dept Physiol & Biophys, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | HISTOLOGY AND HISTOPATHOLOGY; v. 23, n. 11, p. 1367-1377, NOV 2008. |
| Citações Web of Science: | 14 |
| Resumo | |
The growing and indiscriminate use of high doses of anabolic androgenic steroid (AAS) among youth and athletes has raised serious concerns about its hepatotoxic effects. Herein, the influence of AAS in the nuclear phenotype of hepatocytes was investigated in sedentary and trained mice heterozygous for the human CETP (cholesteryl ester transfer protein) transgene and for LDL-receptor null allele (CETP(+/-) LDLr(+/-)) by image analysis. Five groups were assayed comprising treadmill exercised (Ex) and sedentary (Sed) mice, administered mesterolone (AAS) or gum arabic (GA) and a sedentary blank control: G1(SedAAS), G2(SedGA), G3(ExAAS), G4(ExGA), and G5(SedBL). To assess nuclear phenotypes, the state of chromatin supraorganization, DNA content and fragmentation (TUNEL assay), area and perimeter of hepatocytes were determined in Feulgen-stained liver imprints. In addition, the activity of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) hepatic transaminases were measured. SedAAS-G1 showed the lowest chromatin condensation and highest Feulgen-DNA content, polyploid nuclei frequency, nuclear area and perimeter, suggesting gene activation. Contrarily, ExAAS-G3 showed a highest chromatin condensation, and a significant decrease of Feulgen-DNA content and decreased frequency of polyploid nuclei, which suggest gene silencing. Image analysis of the nuclear phenotype offered a coherent descriptive picture of the changing patterns of chromatin organization, which were shown to be congruent with the levels of Feulgen-DNA content, geometric nuclear parameters and hepatocyte activity. In this study, the image analysis permitted the monitoring of the nuclear response to mesterolone and physical exercise action in liver cells, the molecular mechanism of which is in prospect. (AU) | |
| Processo FAPESP: | 04/13768-5 - Influência dos esteróides anabólico-androgênicos (EAA) associados ao exercício físico sobre os músculos de camundongos que expressam proteína de transferência de éster de colesteril (CETP) com deficiência parcial do receptor de LDL-colesterol |
| Beneficiário: | Karina Fontana |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 04/13767-9 - Influência dos esteroides anabólico-androgênicos (EAA) associados ao exercício físico sobre os músculos de camundongos que expressam proteína de transferência de éster de colesteril (CETP) com deficiência parcial do receptor de LDL-colesterol |
| Beneficiário: | Maria Alice da Cruz Hofling |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 03/04597-0 - Ordem molecular e organização supramolecular em feixes de colágeno: diferenças com a funcionalidade, como suposto efeito de expressão de patogenia, e contribuição metodológica |
| Beneficiário: | Benedicto de Campos Vidal |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |