CYCLODEXTRIN GLYCOSYLTRANSFERASE PRODUCTION BY NEW BACILLUS SP STRAINS ISOLATED FROM BRAZILIAN SOIL

Autor(es)

Menocci, Vivian ^[1] ; Goulart, Antonio Jose ^[1] ; Adalberto, Paulo Roberto ^[1] ; Tavano, Olga Luisa ^[1] ; Marques, Daniela Parreira ^[1] ; Contiero, Jonas ^[2] ; Monti, Rubens ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Alimentos & Nutr, BR-14801902 Araraquara, SP - Brazil

^[2] Univ Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, Rio Claro, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 39, n. 4, p. 682-688, OCT-DEC 2008.
• Citações Web of Science: 5

Resumo

Three strains of Bacillus sp. (BACRP, BACNC-1 and BACAR) were isolated from soil adhered to cassava husk. CGTase specific activity for the three isolated strains was higher when cultivated at 40 degrees C. Potato starch, cassava starch, maltodextrin and glucose were used as carbon source and growth temperatures varied from 25 to 55 degrees C. The three isolates presented higher CGTase specific activity when cultivated with potato starch at 40 degrees C. Isolated BACRP and BACAR presented specific activity of 4.0x10(-3) and 2.2x10(-3) U/mg prot at pH 7.0, respectively, when cultivated in mediums added with NaCl 2%; at pH 10,0 their activities were of 3.4x10(-3) and 3.0x10(-3) U/mg prot, respectively, in the same concentration of NaCl. On the other hand, the isolated BACNC-1 presented activity specific of 2.4x10(-3) U/mg prot when cultivated at pH 7.0 added of NaCl 1%, and at pH 10.0 the specific activity was of 3.4x10(-3) U/mg prot without NaCl addition. This work also showed the presence of cyclodextrins formed during fermentation process and that precipitation with acetone or lyophilization followed by dialysis was efficient at removing CDs (cyclodextrins), thus, eliminating interference in the activity assays. The enzyme produced by the BACAR strain was partially purified and beta-CD was liberated as a reaction product. (AU)

• Processo FAPESP: 02/09889-6 - Isolamento de bacterias de solo produtoras de enzima ciclodextrina glucosiltransferase.
• Beneficiário: Vivian Menocci
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica