Genomic instability in blood cells is able to predict the oral cancer risk: an experimental study in rats

Autor(es)

Ribeiro, Daniel A. ^[1] ; Grilli, Daniela G. ^[1] ; Salvadori, Daisy M. F. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Biosci, BR-11060001 Santos, SP - Brazil

^[2] UNESP, Sao Paulo State Univ, Dept Pathol, Botucatu Med Sch, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Molecular Histology; v. 39, n. 5, p. 481-486, OCT 2008.
• Citações Web of Science: 28

Resumo

This study was undertaken to investigate the genomic instability on blood cells during 4-nitroquinoline 1-oxide (4NQO)-induced rat tongue carcinogenesis by means of single cell gel (comet) and micronucleus assays. Male Wistar rats were distributed into three groups of 10 animals each and treated with 50 ppm 4NQO solution through their drinking water for 4, 12, and 20 weeks. Ten animals were used as negative control. Although no histopathological abnormalities were induced in the epithelium after 4 weeks of carcinogen exposure, genetic damage was found in blood cells as depicted by the mean tail moment and an increase of micronucleated polychromatic erythrocytes. After 12 and 20 weeks treatment, the same picture occurred, being the strong effect observed in the micronucleus induction. These periods correspond to pre-neoplastic lesions and well-differentiated squamous cell carcinomas, respectively. Taken together, our results support the idea that genomic instability on blood cells appears to be associated with the risk and progression of oral cancer, being a reliable tool for detecting early systemic conditions of malignancy. (AU)

• Processo FAPESP: 07/01228-4 - Carcinogênese bucal quimicamente induzida pela 4-nitroquinolina 1-óxido em ratos: possíveis biomarcadores envolvidos em sua patogênese
• Beneficiário: Daniel Araki Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores