Changes in types of muscle fibers induced by transcutaneous electrical stimulation of the diaphragm of rats

Autor(es)

Costa, D. ^{[1, 2]} ; Cancelliero, K. M. ^[1] ; Campos, G. E. R. ^{[3, 2]} ; Salvini, T. F. ^[1] ; da Silva, C. A. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Fisioterapia, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Metodista Piracicaba, Dept Fisioterapia, Piracicaba, SP - Brazil

^[3] Univ Estadual Campinas, Dept Anat, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 41, n. 9, p. 809-811, SEP 2008.
• Citações Web of Science: 2

Resumo

The objective of the present study was to assess the effect of transcutaneous electrical diaphragmatic stimulation (TEDS) on different types of diaphragm muscle fibers. Male Wistar rats (8-12 weeks old) were divided into 2 experimental groups (N = 8 in each group): 1) control, 2) animals submitted to TEDS [frequency = 50 Hz; T ON/T OFF (contraction/relaxation time) = 2/2 s; pulse duration = 0.4 ms, intensity = 5 mA with a 1 mA increase every 3 min for 20 min] for 7 days. After completing this treatment period, the I, IIA, IIB, and IID diaphragm muscle fibers were identified using the mATPase technique. Statistical analysis consisted of the normality, homoscedasticity and t-tests (P < 0.05). There was a 19.6% (P < 0.05) reduction in the number of type I fibers and a 49.7% increase (P < 0.05) in type IID fibers in the TEDS group compared with the control group. An important result of the present study was that electrical stimulation with surface electrodes was efficient in altering the distribution of fibers in diaphragm muscle. This therapeutic resource could be used in the treatment of respiratory muscle alterations. (AU)

• Processo FAPESP: 04/14798-5 - A influencia da estimulacao diafragmatica eletrica transcutanea em ratos tratados com bleomicina.
• Beneficiário: Karina Maria Cancelliero
• Modalidade de apoio: Bolsas no Brasil - Doutorado