Endostatin, an antiangiogenic protein, is expressed in the unilateral ureteral obstruction mice model

Autor(es)

Maciel, Thiago T. ^[1] ; Coutinho, Enia L. ^[1] ; Soares, Debora ^[1] ; Achar, Eduardo ^[1] ; Schor, Nestor ^[1] ; Bellini, Maria H. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Div Nephrol, BR-04023900 Sao Paulo - Brazil

^[2] IPEN CNEN SP, Ctr Quim & Meio Ambiente, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NEPHROLOGY; v. 21, n. 5, p. 753-760, SEP-OCT 2008.
• Citações Web of Science: 14

Resumo

Background: Extracellular matrix accumulation, epithelial-to-mesenchymal transition, tubular atrophy and loss of peritubular capillary network are hallmarks of tubulointerstitial injury in progressive renal diseases. In this study, we analyzed endostatin expression in kidneys subjected to unilateral ureteral obstruction (UUO). Methods: Collagen XVIII mRNA expression was evaluated by real-time polymerase chain reaction (PCR). Endostatin and CD31 protein levels were analyzed by Western blot and immunohistochemistry. In vitro quantification of collagen XVIII and fibrosis-related genes in HK2 cells was performed by real-time PCR. Results: UUO significantly increased collagen XVIII mRNA expression and released a 30-kDa endostatin fragment. Immunohistochemistry revealed endostatin expression increased in injured tissue, mainly on tubular cells. Of interest, expression of CD31 was significantly reduced by UUO. Endostatin administration in vitro did not modify the expression of genes related to fibrosis development. However, in vitro TGF-b1 administration induced expression of collagen XVIII/endostatin mRNA in human tubular cells. Conclusion: Endostatin is expressed during the progression of renal fibrosis in vitro and in vivo, suggesting a role for endostatin in development of tubulointerstitial injury. (AU)

• Processo FAPESP: 04/08311-6 - Mecanismos moleculares, celulares e fisiopatológicos da insuficiência renal aguda
• Beneficiário: Nestor Schor
• Modalidade de apoio: Auxílio à Pesquisa - Temático