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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Expression analysis of putative vitellogenin and lipophorin receptors in honey bee (Apis mellifera L.) queens and workers

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Autor(es):
Guidugli-Lazzarini, Karina Rosa [1] ; do Nascimento, Adriana Mendes [1] ; Tanaka, Erica Donato [1] ; Dolors Piulachs, Maria [2] ; Hartfelder, Klaus [3] ; Bitondi, Marcia Gentile [1] ; Simoes, Zila Luz Paulino [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-05508 Sao Paulo - Brazil
[2] CSIC, Inst Mol Biol Barcelona, Dept Physiol & Mol Biodivers, Barcelona - Spain
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Patogenicos, Ribeirao Preto, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF INSECT PHYSIOLOGY; v. 54, n. 7, p. 1138-1147, JUL 2008.
Citações Web of Science: 42
Resumo

Two members of the low density lipoprotein receptor (LDLR) family were identified as putative orthologs for a vitellogenin receptor (Amvgr) and a lipophorin receptor (Amlpr) in the Apis mellifera genome. Both receptor sequences have the structural motifs characteristic of LDLR family members and show a high degree of similarity with sequences of other insects. RT-PCR analysis of Amvgr and Amlpr expression detected the presence of both transcripts in different tissues of adult female (ovary, fat body, midgut, head and specifically hypopharyngeal gland), as well as in embryos. In the head RNA samples we found two variant forms of AmLpR: a full length one and a shorter one lacking 29 amino acids in the O-linked sugar domain. In ovaries the expression levels of the two honey bee LDLR members showed opposing trends: whereas Amvgr expression was upregulated as the ovaries became activated, Amlpr transcript levels gradually declined. In situ hybridization analysis performed on ovaries detected Amvgr mRNA exclusively in germ line cells and corroborated the qPCR results showing an increase in Amvgr gene expression concomitant with follicle growth. (C) 2008 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 05/03926-5 - Genômica funcional de Apis mellifera: busca de novos genes e redes funcionais no contexto do desenvolvimento, da diferenciação de castas e da reprodução
Beneficiário:Zilá Luz Paulino Simões
Linha de fomento: Auxílio à Pesquisa - Temático