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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Expression analysis of putative vitellogenin and lipophorin receptors in honey bee (Apis mellifera L.) queens and workers

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Author(s):
Guidugli-Lazzarini, Karina Rosa [1] ; do Nascimento, Adriana Mendes [1] ; Tanaka, Erica Donato [1] ; Dolors Piulachs, Maria [2] ; Hartfelder, Klaus [3] ; Bitondi, Marcia Gentile [1] ; Simoes, Zila Luz Paulino [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-05508 Sao Paulo - Brazil
[2] CSIC, Inst Mol Biol Barcelona, Dept Physiol & Mol Biodivers, Barcelona - Spain
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Patogenicos, Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF INSECT PHYSIOLOGY; v. 54, n. 7, p. 1138-1147, JUL 2008.
Web of Science Citations: 42
Abstract

Two members of the low density lipoprotein receptor (LDLR) family were identified as putative orthologs for a vitellogenin receptor (Amvgr) and a lipophorin receptor (Amlpr) in the Apis mellifera genome. Both receptor sequences have the structural motifs characteristic of LDLR family members and show a high degree of similarity with sequences of other insects. RT-PCR analysis of Amvgr and Amlpr expression detected the presence of both transcripts in different tissues of adult female (ovary, fat body, midgut, head and specifically hypopharyngeal gland), as well as in embryos. In the head RNA samples we found two variant forms of AmLpR: a full length one and a shorter one lacking 29 amino acids in the O-linked sugar domain. In ovaries the expression levels of the two honey bee LDLR members showed opposing trends: whereas Amvgr expression was upregulated as the ovaries became activated, Amlpr transcript levels gradually declined. In situ hybridization analysis performed on ovaries detected Amvgr mRNA exclusively in germ line cells and corroborated the qPCR results showing an increase in Amvgr gene expression concomitant with follicle growth. (C) 2008 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 05/03926-5 - Functional genomics of Apis mellifera: search for new genes and functional networks in the context of development, different castes and reproduction
Grantee:Zilá Luz Paulino Simões
Support Opportunities: Research Projects - Thematic Grants