| Texto completo | |
| Autor(es): |
Conte, T. C.
[1]
;
Franco, D. V.
[2]
;
Baptista, I. L.
[3]
;
Bueno, Jr., C. R.
[4]
;
Selistre-de-Araujo, H. S.
[5]
;
Brum, P. C.
[4]
;
Moriscot, A. S.
[3]
;
Miyabara, E. H.
[2]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Anat, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Phys Therapy, BR-03071000 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, BR-05508900 Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Biodynam, Sch Phys Educ & Sport, BR-05508900 Sao Paulo - Brazil
[5] Univ Fed Sao Carlos, Dept Physiol, BR-13565905 Sao Carlos, SP - Brazil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Toxicon; v. 52, n. 1, p. 146-155, JUL 2008. |
| Citações Web of Science: | 8 |
| Resumo | |
This work investigates the influence of heat shock proteins (HSPs) on necrosis and subsequent skeletal muscle regeneration induced by crotoxin (CTX), the major component of Crotalus durissus terrificus venom. Mice were treated with radicicol, a HSP inductor, followed by an intramuscular injection of CTX into the gastrocnemius muscle. Treated groups were sacrificed 1, 10 and 21 days after CTX injection. Muscle histological sections were stained with toluidine blue and assayed for acid phosphatase or immunostained with either neuronal cell adhesion molecule (NCAM) or neonatal myosin heavy chain (MHCn). Muscle samples were also submitted to Western blotting analysis. The results show that CTX alone and CTX combined with radicicol induced a similar degree of myofiber necrosis. CTX-injured muscles treated with radicicol had increased cross-sectional areas at 10 and 21 days post-lesion compared with untreated CTX-injured muscles. Additionally, radicicol significantly increased the number of NCAM-positive satellite cells in the gastrocnemius at one day post-CTX injury. CTX-injured Muscles treated with radicicol contained more MHCn-positive regenerating myofibers compared with untreated CTX-injured muscles. These results suggest that HSPs contribute to the regeneration of myofibers damaged by CTX. Additionally, further studies should investigate the potential therapeutic effects of radicicol in skeletal muscles affected by Crotalus venom. (C) 2008 Elsevier Ltd. All rights reserved. (AU) | |