Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Nutritional stress enhances cell viability of odontoblast-like cells subjected to low level laser irradiation

Texto completo
Autor(es):
Tagliani, M. M. [1] ; Oliveira, C. F. [1] ; Lins, E. M. M. [2] ; Kurachi, C. [2] ; Hebling, J. [1] ; Bagnato, V. S. [2] ; de Souza Costa, C. A. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] UNESP, Araraquara Sch Dent, BR-14801903 Araraquara, SP - Brazil
[2] Univ Sao Paulo, Inst Phys, BR-13560970 Sao Carlos, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: LASER PHYSICS LETTERS; v. 7, n. 3, p. 247-251, MAR 2010.
Citações Web of Science: 35
Resumo

In spite of knowing that cells tinder stress are biostimulated by low level laser (LLL) irradiation, the ideal condition of stress to different cell lines has not yet been established. Consequently, the aim of the present in vitro study was to evaluate the effects of a defined parameter of LLL irradiation applied on stressed odontoblast-like pulp cells (MDPC-23). The cells were seeded (12500 cells/cm(2)) in wells of 24-well plates using complete culture medium (DMEM) and incubated for 24 hours. Then, the DMEM was replaced by a new medium with low concentrations (nutritional stress condition) of fetal bovine serum (FBS) giving rise to the following experimental groups: G1: 2% FBS; G2: 5% FBS; and G3: 10% FBS. The cells were irradiated three times with LLL in specific parameters (808 +/- 3 nm, 100 mW, 1.5 J/cm(2)) every 24 hours. No irradiation was carried out in groups G4 (2% FBS-Control), G5 (5% FBS-Control), and G6 (10% FBS-Control). For all groups, the cell metabolism (MTT assay) and morphology (SEM) was evaluated. The experimental groups showed enhanced cell metabolism and normal cell morphology regardless of FBS concentration. A slight increase in the cell metabolism was observed only in group G2. It was concluded that cell nutritional stress caused by reducing the concentration of FBS to 5% is the most suitable method to assess the biostimulation of LLL irradiated MDPC-23 cells. {[}GRAPHICS] MDPC-23 cells adhered to the glass substrate exhibited a spindle-shaped morphology and some mitosis were observed (arrows); SEM x500 (C) 2010 by Astro Ltd. Published exclusively by WILEY-VCH Verlag GmbH \& Co. KGaA (AU)

Processo FAPESP: 07/50646-3 - Avaliação do efeito bioestimulador direto e transdentinário do laser de baixa potência sobre células odontoblastóides em cultura
Beneficiário:Carlos Alberto de Souza Costa
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/08424-6 - Efeito direto e transdentinário do LED 660 nm sobre células de linhagem odontoblástica
Beneficiário:Carlos Alberto de Souza Costa
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/54785-0 - Efeito do laser de baixa intensidade sobre celulas odontoblastoides in vitro e complexo dentino-pulpar in vivo
Beneficiário:Camila Fávero de Oliveira
Linha de fomento: Bolsas no Brasil - Doutorado