Comparison between RT-PCR and the mouse inoculation test for detection of rabies virus in samples kept for long periods under different conditions

Autor(es)

Lopes, Marissol Cardoso ^[1] ; Rossignolo Venditti, Leandro Lima ^[1] ; Queiroz, Luzia Helena ^[1]

Afiliação do(s) autor(es)

^[1] UNESP, Prod & Anim Hlth Dept, BR-16050680 Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Virological Methods; v. 164, n. 1-2, p. 19-23, MAR 2010.
• Citações Web of Science: 9

Resumo

Antigenic and genetic analyses are important tools for retrospective studies of rabies epidemiology in specific geographical areas. Virus recovery and re-isolation from archival samples conserved for long periods at freezing temperature are essential for these studies. Prolonged preservation, associated with temperature variations, causes significant loss of virus viability. However, molecular tools, such as RT-PCR, can overcome this problem. For this purpose, 95 positive samples stored for 4-13 years at -20 and -80 degrees C were evaluated by mouse inoculation test and RT-PCR. Only 32 (33.6%) of the samples were positive with the mouse inoculation test, while RT-PCR detected the viral genome in 62 (65.3%) samples. When the samples were analyzed in relation to storage period, there was a significant difference in those stored for >10 years, with 59.7% positivity for RT-PCR and 22.1% for mouse inoculation test. The present study confirms the significance of RT-PCR for detection of viral genomes in archival samples, including those in an apparent state of decomposition. (C) 2009 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 04/06740-7 - Estudo antigênico e genético de isolados do vírus rábico na região de Araçatuba, São Paulo, no período de 1993 a 2003
• Beneficiário: Luzia Helena Queiroz
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 04/12792-0 - Avaliação da viabilidade do vírus rábico em amostras positivas de cérebro, de diferentes espécies animais, conservadas em temperatura de congelamento por longo período de tempo
• Beneficiário: Leandro Lima Rossignolo Venditti
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 04/12793-6 - Detecção do genoma do vírus rábico, pela técnica de RT-PCR, em cérebro de diferentes espécies animais, conservados em temperatura de congelamento por longo período de tempo
• Beneficiário: Marissol Cardoso Lopes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica