| Texto completo | |
| Autor(es): |
Fontana, Karina
[1, 2]
;
Almeida, Fernanda M.
[3]
;
Tomiosso, Tatiana Carla
[3]
;
Pimentel, Edson Rosa
[3]
;
da Cruz Hofling, Maria Alice
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Campinas UNICAMP, Dept Histol & Embryol, Inst Biol, BR-13083970 Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Fac Med Sci, Dept Pharmacol, BR-13083970 Campinas - Brazil
[3] Univ Campinas UNICAMP, Dept Anat Cell Biol & Physiol & Biophys, BR-13083970 Campinas - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Cell and Tissue Research; v. 339, n. 2, p. 411-420, FEB 2010. |
| Citações Web of Science: | 5 |
| Resumo | |
The effect of mesterolone and intensive treadmill training (6 weeks, 5 days/week, means: 15.82 m/min and 45.8 min/day) in Achilles tendon remodeling was evaluated. Sedentary mice treated with mesterolone (Sed-M) or vehicle (Sed-C, placebo/control) and corresponding exercised (Ex-M and Ex-C) were examined. SDS-polyacrylamide gel electrophoresis was used for determining collagen bands and hydroxyproline concentration. Collagen fibril diameter, the area and number of fibrils contained in an area probe, and the ultrastructure of fibroblasts (tenocytes) were determined. The presence of collagen was notable in the tendons of all groups. Collagen alpha(1/)alpha(2) bands in Sed-M, Ex-C, and Ex-M were higher than in Sed-C, as shown by hydroxyproline content, but collagen beta-chain appeared only in Ex-C. Noticeable bands of non-collagenous proteins were found in Sed-M and Ex-M. The number of fibrils in the area probe increased markedly in Sed-M and Ex-C (12-fold), but their diameter and area were unchanged compared with Sed-C. In Ex-M, the fibril number decreased by three-fold to 3.5-fold compared with Sed-M and Ex-C, whereas diameter and area increased. Sed-C tenocytes appeared quiescent, whereas those in the other groups seemed to be engaged in protein synthesis. The density of tenocytes was smaller in Sed-C than in Ex-C, Sed-M, and Ex-M. Thus, mechanical stimuli and mesterolone alter the morphology of tenocytes and the composition of the tendon, probably through fibrillogenesis and/or increased intermolecular cross-links. The ergogenic effect is evidenced by the activation of collagenous and non-collagenous protein synthesis and the increase in the diameter and area of collagen fibrils. This study might be relevant to clinical sports medicine. (AU) | |
| Processo FAPESP: | 04/13767-9 - Influência dos esteroides anabólico-androgênicos (EAA) associados ao exercício físico sobre os músculos de camundongos que expressam proteína de transferência de éster de colesteril (CETP) com deficiência parcial do receptor de LDL-colesterol |
| Beneficiário: | Maria Alice da Cruz Hofling |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 04/13768-5 - Influência dos esteróides anabólico-androgênicos (EAA) associados ao exercício físico sobre os músculos de camundongos que expressam proteína de transferência de éster de colesteril (CETP) com deficiência parcial do receptor de LDL-colesterol |
| Beneficiário: | Karina Fontana |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |