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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Validated spectrophotometric and spectrofluorimetric methods for determination of chloroaluminum phthalocyanine in nanocarriers

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Autor(es):
Siqueira-Moura, M. P. [1] ; Primo, F. L. [1] ; Peti, A. P. F. [2] ; Tedesco, A. C. [2]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, FCFRP, Programa Posgrad Ciencias Farmaceut, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, FFCLRP, Lab Fotobiol & Fotomed, Dept Quim, Ribeirao Preto, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Pharmazie; v. 65, n. 1, p. 9-14, JAN 2010.
Citações Web of Science: 20
Resumo

UV-VIS-Spectrophotometric and spectrofluorimetric methods have been developed and validated allowing the quantification of chloroaluminum phthalocyanine (CIAIPc) in nanocarriers. In order to validate the methods, the linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, and selectivity were examined according to USP 30 and ICH guidelines. Linearities range were found between 0.50-3.00 mu g.mL(-1) (Y=0.3829 X {[}CIAIPc, mu g.mL(-1)] + 0.0126; r=0.9992) for spectrophotometry, and 0.05-1.00 mu g.mL(-1) (Y=2.24 x 10(6) X {[}CIAIPc, mu g.L(-1)] + 9.74 x 10(4); r=0.9978) for spectrofluorimetry. In addition, ANOVA and Lack-of-fit tests demonstrated that the regression equations were statistically significant (p<0.05), and the resulting linear model is fully adequate for both analytical methods. The LOD values were 0.09 and 0.01 mu g.mL(-1), while the LOCI were 0.27 and 0.04 mu g.mL(-1) for spectrophotometric and spectrofluorimetric methods, respectively. Repeatability and intermediate precision for proposed methods showed relative standard deviation (RSD) between 0.58% to 4.80%. The percent recovery ranged from 98.9% to 102.7% for spectrophotometric analyses and from 94.2% to 101.2% for spectrofluorimetry. No interferences from common excipients were detected and both methods were considered specific. Therefore, the methods are accurate, precise, specific, and reproducible and hence can be applied for quantification of CIAIPc in nanoemulsions (NE) and nanocapsules (NC). (AU)

Processo FAPESP: 07/58809-9 - Nanocarreadores contendo ftalocianina de cloroalumínio: desenvolvimento, caracterização físico-química e avaliação in vitro da fotocitotoxicidade em melanoma
Beneficiário:Marigilson Pontes de Siqueira Moura
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 06/50562-1 - Avaliação do uso de laser de baixa potência e agentes fotossensibilizadores associados a sistemas de liberação nanoestruturados no tratamento de feridas e/ou outras doenças cutâneas
Beneficiário:Fernando Lucas Primo
Linha de fomento: Bolsas no Brasil - Doutorado