PHA(MCL) biosynthesis systems in Pseudomonas aeruginosa and Pseudomonas putida strains show differences on monomer specificities

Autor(es)

Silva-Queiroz, S. R. ^{[1, 2]} ; Silva, L. F. ^[1] ; Pradella, J. G. C. ^{[1, 2]} ; Pereira, E. M. ^{[1, 2]} ; Gomez, J. G. C. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Inst Butantan, IPT, Programa Posgrad Interunidades Biotecnol USP, Sao Paulo - Brazil

^[2] Inst Pesquisas Tecnol Sao Paulo, Ctr Tecnol Proc Prod, Lab Biotecnol Ind, IPT, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biotechnology; v. 143, n. 2, p. 111-118, AUG 20 2009.
• Citações Web of Science: 21

Resumo

The production of PHA from plant oils by Pseudomonas species soil isolated from a sugarcane crop was evaluated. Out of 22 bacterial strains three were able to use efficiently plant oils to grow and to accumulate PHA. Pseudomonas putida and Pseudomonas aeruginosa strains produced PHA presenting differences on monomer composition compatible with variability on monomer specificity of their PHA biosynthesis system. The molar fraction of 3-hydroxydodecanoate detected in the PHA was linearly correlated to the oleic acid supplied. A non-linear relationship between the molar fractions of 3-hydroxy-6-dodecenoate (3HDd Delta(6)) detected in PHA and the linoleic acid supplied was observed, compatible with saturation in the biosynthesis system capability to channel intermediate of P-oxidation to PHA synthesis. Although P. putida showed a higher 3HDd Delta(6) yield from linoleic acid when compared to P. aeruginosa, in both species it was less than 10% of the maximum theoretical value. These results contribute to the knowledge about the biosynthesis of PHA with a controlled composition from plant oils allowing in the future establishing the production of these polyesters as tailor-made polymers. (C) 2009 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 03/01602-2 - Avaliação do metabolismo de síntese de PHAMCL e controle de sua composição monomérica
• Beneficiário: José Gregório Cabrera Gomez
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 00/00689-9 - Obtencao de mutantes deficientes na oxidacao de acidos graxos insaturados para producao de elastomeros biodegradaveis.
• Beneficiário: Sonia Regina da Silva Queiroz
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 03/11795-2 - Estudo do metabolismo de ácidos graxos em Pseudomonas putida visando a modulação da composição monomérica de elastômero biodegradável
• Beneficiário: Sonia Regina da Silva Queiroz
• Modalidade de apoio: Bolsas no Brasil - Doutorado