| Full text | |
| Author(s): |
Silva-Queiroz, S. R.
[1, 2]
;
Silva, L. F.
[1]
;
Pradella, J. G. C.
[1, 2]
;
Pereira, E. M.
[1, 2]
;
Gomez, J. G. C.
[1, 2]
Total Authors: 5
|
| Affiliation: | [1] Inst Butantan, IPT, Programa Posgrad Interunidades Biotecnol USP, Sao Paulo - Brazil
[2] Inst Pesquisas Tecnol Sao Paulo, Ctr Tecnol Proc Prod, Lab Biotecnol Ind, IPT, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Journal of Biotechnology; v. 143, n. 2, p. 111-118, AUG 20 2009. |
| Web of Science Citations: | 21 |
| Abstract | |
The production of PHA from plant oils by Pseudomonas species soil isolated from a sugarcane crop was evaluated. Out of 22 bacterial strains three were able to use efficiently plant oils to grow and to accumulate PHA. Pseudomonas putida and Pseudomonas aeruginosa strains produced PHA presenting differences on monomer composition compatible with variability on monomer specificity of their PHA biosynthesis system. The molar fraction of 3-hydroxydodecanoate detected in the PHA was linearly correlated to the oleic acid supplied. A non-linear relationship between the molar fractions of 3-hydroxy-6-dodecenoate (3HDd Delta(6)) detected in PHA and the linoleic acid supplied was observed, compatible with saturation in the biosynthesis system capability to channel intermediate of P-oxidation to PHA synthesis. Although P. putida showed a higher 3HDd Delta(6) yield from linoleic acid when compared to P. aeruginosa, in both species it was less than 10% of the maximum theoretical value. These results contribute to the knowledge about the biosynthesis of PHA with a controlled composition from plant oils allowing in the future establishing the production of these polyesters as tailor-made polymers. (C) 2009 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 03/11795-2 - Study of fatty acids metabolism in Pseudomonas putida aiming to modulate monomer composition of biodegradable elastomer |
| Grantee: | Sonia Regina da Silva Queiroz |
| Support Opportunities: | Scholarships in Brazil - Doctorate |