Cloning and overexpression of the xylose isomerase gene from Burkholderia sacchari and production of polyhydroxybutyrate from xylose

Autor(es)

Garcez Lopes, Mateus Schreiner ^[1] ; Cabrera Gomez, Jose Gregorio ^[1] ; Silva, Luiziana Ferreira ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Microbiol, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Canadian Journal of Microbiology; v. 55, n. 8, p. 1012-1015, AUG 2009.
• Citações Web of Science: 6

Resumo

A different organization for the xyl operon was found in different genomes of Burkholderia and Pseudomomas species. Degenerated primers were designed based on Burkholderia genomes and used to amplify the xylose isomerase gene (xylA) from Burkholderia sacchari IPT101 The gene encoded a protein of 329 amino acids, which showed the highest similarity (90%) to the homologous gene of Burkholderia dolosa. It was cloned in the broad host range plasmid pBBR1MCS-2, which partially restored growth and polyhydroxybutyrate production capability in xylose to a B. sacchari xyl(-) mutant. When xylA was overexpressed in the wild-type strain, it was not able to increase growth and polyhydroxybutyrate production, suggesting that XylA activity is not limiting for xylose utilization in B. sacchari. (AU)

• Processo FAPESP: 04/15369-0 - Construções de linhagens recombinantes para a produção de plástico biodegradável a partir de hidrolisado de bagaço de cana-de-açúcar
• Beneficiário: Mateus Schreiner Garcez Lopes
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto