| Texto completo | |
| Autor(es): |
Koide, Tie
[1]
;
Silva Neto, José F. da
;
Gomes, Suely Lopes
;
Marques, Marilis V.
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Universidade de São Paulo (USP). Instituto de Química. Departamento de Bioquímica - Brasil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Current Microbiology; v. 48, n. 4, p. 247-250, Apr. 2004. |
| Área do conhecimento: | Ciências Biológicas - Genética |
| Assunto(s): | Xylella fastidiosa Mutagênese Recombinases Transposon resolvases |
| Resumo | |
To overcome the difficulty in obtaining mutants of the citrus strains of Xylella fastidiosa, we evaluated mutagenesis using the transposome system as a tool for the isolation of a large number of mutants. Electroporation of a commercial transposome system in X. fastidiosa CVC (Citrus Variegated Chlorosis) strain J1a12 yielded an efficiency of 1.2 × 103 kanamycin (Km)-resistant clones per g of DNA. Southern blot analysis demonstrated that the transposon was randomly inserted, and nucleotide sequence analysis indicated the presence of 9 bp direct repeats flanking the transposon insertion site. Analysis by PCR of one of the insertion mutants (clone J15) showed that the transposon was stable after eight passages in solid media. These results show that the transposome system can be used to generate a random mutant library of Xylella fastidiosa CVC strain. (AU) | |
| Processo FAPESP: | 98/16251-0 - Establishment of conditions for the disruption genes potentially involved in Xylella fastidiosa pathogenesis |
| Beneficiário: | Suely Lopes Gomes |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa GENOMA |