Histological evaluation of biocompatibility of lyophilized bovine pericardium implanted subcutaneously in rats

Autor(es)

Maizato‚ M.J.S. ; Pires‚ M.D. ; Canzian‚ M. ; Higa‚ O.Z. ; Pitombo‚ R.N.M. ; Leirner‚ A.A.

• Tipo de documento: Artigo Científico
• Fonte: ARTIFICIAL ORGANS; v. 32, n. 4, p. 268-271, 2008.

Resumo

This article aims at investigating in vivo evaluation of lyophilization procedure on the biocompatibility of bovine pericardium treated with glutaraldehyde (GA). The bovine pericardium was fixed with 0.5% glutaraldehyde during 10 days and preserved in 4% formaldehyde (FA). Two groups of samples were prepared from treated membranes: Group 1, nonlyophilized samples and Group 2, lyophilized samples. Male Sprague-Dawley rats (4 weeks after birth) were anesthetized (pentobarbital sodium 25 mg/kg of body weight) and in each one were implanted subcutaneously in the dorsal region a sample from Group 1 and another from Group 2. These samples were explanted after 30 days for histological analysis. No intercurrences took place after the surgery. No differences (P > 0.05) in the calcification, granulomatous reaction, mononuclear infiltration, and granulation tissue development was observed between both groups. The implanted lyophilized samples presented a trend for a reduced inflammatory reaction. Lyophilization of the bovine pericardium does not seem to increase the above listed tissue reaction. (AU)

• Processo FAPESP: 04/09566-8 - Liofilização de tecidos biológicos: pesquisa sob ponto de vista do desenvolvimento de biomateriais
• Beneficiário: Ronaldo Nogueira de Moraes Pitombo
• Modalidade de apoio: Auxílio à Pesquisa - Temático