Histological evaluation of biocompatibility of lyophilized bovine pericardium implanted subcutaneously in rats

Author(s)

Maizato‚ M.J.S. ; Pires‚ M.D. ; Canzian‚ M. ; Higa‚ O.Z. ; Pitombo‚ R.N.M. ; Leirner‚ A.A.

• Document type: Journal article
• Source: ARTIFICIAL ORGANS; v. 32, n. 4, p. 268-271, 2008.

Abstract

This article aims at investigating in vivo evaluation of lyophilization procedure on the biocompatibility of bovine pericardium treated with glutaraldehyde (GA). The bovine pericardium was fixed with 0.5% glutaraldehyde during 10 days and preserved in 4% formaldehyde (FA). Two groups of samples were prepared from treated membranes: Group 1, nonlyophilized samples and Group 2, lyophilized samples. Male Sprague-Dawley rats (4 weeks after birth) were anesthetized (pentobarbital sodium 25 mg/kg of body weight) and in each one were implanted subcutaneously in the dorsal region a sample from Group 1 and another from Group 2. These samples were explanted after 30 days for histological analysis. No intercurrences took place after the surgery. No differences (P > 0.05) in the calcification, granulomatous reaction, mononuclear infiltration, and granulation tissue development was observed between both groups. The implanted lyophilized samples presented a trend for a reduced inflammatory reaction. Lyophilization of the bovine pericardium does not seem to increase the above listed tissue reaction. (AU)

• FAPESP's process: 04/09566-8 - Liofilization of biological tissue: research from the point of view of development of biomaterials
• Grantee: Ronaldo Nogueira de Moraes Pitombo
• Support Opportunities: Research Projects - Thematic Grants