Apoptosis and extracellular matrix remodelling in human silicosis

Autor(es)

Delgado‚ L. ; Parra‚ ER ; Capelozzi‚ VL

• Tipo de documento: Artigo Científico
• Fonte: Histopathology; v. 49, n. 3, p. 283-289, 2006.

Resumo

Aims: Silicosis is a chronic occupational disease caused by the inhalation of free crystalline silica particles which produce inflammation and tissue destruction followed by remodelling of the extracellular matrix. Apoptosis has been implicated in the development of the initial inflammation that triggers the remodelling process. Our aim was to elucidate the importance of Fas-ligand (Fas-L) in this disorder and to study the relationship between Fas-L and several other inflammatory and fibrotic remodelling markers. Methods and results: We analysed 23 lung biopsies from silicotic patients and five controls, quantifying Fas-L and Bcl-2 expression by inflammatory cells as well as mast cells and collagen and elastic fibres. We used immunohistochemistry and morphometry to evaluate the amount of Fas-L and Bcl-2. Our analysis revealed that the silicotic lung stage was significantly related to Fas-L, mast cell and extracellular matrix remodelling. Fas-L expression was inversely associated with mast cells, collagen/elastic deposition and the silicotic lung. Conclusion: Fas-L, mast cell staining and collagen/elastic fibre quantities in silicotic lungs are strongly related to silicosis progression. (AU)

• Processo FAPESP: 01/14566-9 - Determinantes clinicos, funcionais e histopatológicos de prognóstico nas pneumonias intersticiais idiopaticas com ênfase ao componente vascular
• Beneficiário: Edwin Roger Parra Cuentas
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 03/00162-9 - Pneumonia intersticial idiopatica (iip): estudo das moleculas de adesao.
• Beneficiário: Ligia Rodrigues Silverio da Costa
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 00/14336-0 - Doenças pulmonares intersticiais (ILD): correlação morfo-tomográfica através de métodos histoquímicos, imunohistoquímicos e morfométricos
• Beneficiário: Vera Luiza Capelozzi
• Modalidade de apoio: Auxílio à Pesquisa - Temático