In vitro efficacy of the essential oil of Piper cubeba L. (Piperaceae) against Schistosoma mansoni

Autor(es):Mostrar menos -

Magalhaes, Lizandra G. ^[1] ; de Souza, Julia M. ^[1] ; Wakabayashi, Kamila A. L. ^[1] ; Laurentiz, Rosangela da S. ^{[2, 3]} ; Vinholis, Adriana H. C. ^[1] ; Rezende, Karen C. S. ^[1] ; Simaro, Guilherme V. ^[1] ; Bastos, Jairo K. ^{[4, 5]} ; Rodrigues, Vanderlei ^{[6, 7]} ; Esperandim, Viviane R. ^[1] ; Ferreira, Daniele S. ^[1] ; Crotti, Antonio E. M. ^[1] ; Cunha, Wilson R. ^[1] ; Silva, Marcio L. A. E. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Franca, Nucleo Pesquisas Ciencias Exatas & Tecnol, BR-14404600 Franca, SP - Brazil

^[2] Faculdade Engenharia Ilha Solteira, Univ Estadual Paulista, Dept Quim Fis, Avenida Brasil 56, Ilha Solteira, SP - Brazil

^[3] Univ Estadual Paulista, Dept Quim & Fis, Fac Engn Ilha Solteira, BR-15385000 Ilha Solteira, SP - Brazil

^[4] Faculdade Farm Ribeiro Preto, Univ So Paulo, Dept Cincias Farmacut, Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Dept Ciencias Farmaceut, Fac Farm Ribeirao Preto, BR-14030000 Ribeirao Preto, SP - Brazil

^[6] Univ Sao Paulo, Dept Bioquim & Imunol, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

^[7] Faculdade Medicina Ribeiro Preto, Univ So Paulo, Dept Bioquim Imunologia, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Parasitology Research; v. 110, n. 5, p. 1747-1754, MAY 2012.
• Citações Web of Science: 29

Resumo

In this paper, cercariae, schistosomula, and adult Schistosoma mansoni worms were incubated in vitro with the essential oil of Piper cubeba (PC-EO) at concentrations from 12.5 to 200 mu g/mL, and the viability was evaluated using an inverted microscopy. The effects of PC-EO at 100 and 200 mu g/mL on the stages of S. mansoni were similar to those of the positive control (PZQ at 12.5 mu g/mL), with total absence of mobility after 120 h. However, at concentrations from 12.5 to 50 mu g/mL, PC-EO caused a reduction in the viability of cercariae and schistosomula when compared with the negative control groups (RPMI 1640 or dechlorinated water) or (RPMI 1640 + 0.1% DMSO or dechlorinated water + 0.1% DMSO). On the other hand, adult S. mansoni worms remained normally active when incubated with PC-EO at concentrations of 12.5 and 25 mu g/mL, and their viabilities were similar to those of the negative control groups. In addition, at concentrations ranging from 50 to 200 mu g/mL, separation of all the coupled adult worms was observed after 24 h of incubation, which is related to the fact of the reduction in egg production at this concentration. The main chemical constituents of PC-EO were identified by gas chromatography-mass spectrometry as being sabinene (19.99%), eucalyptol (11.87%), 4-terpineol (6.36%), beta-pinene (5.81%), camphor (5.61%), and delta-3-carene (5.34%). The cytotoxicity of the PC-EO was determined, and a significant cytotoxicity was only obtained in the concentration of 200 mu g/mL after 24 h treatment. The results suggest that PC-EO possesses an effect against cercariae, schistosomula, and adult worms of the S. mansoni. (AU)

• Processo FAPESP: 09/15207-4 - Avaliação do efeito de lignanas e neolignanas em Schistosoma mansoni: uma abordagem proteômica
• Beneficiário: Lizandra Guidi Magalhães
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado