DEVELOPMENT OF MICROSATELLITE MARKERS FOR ANADENANTHERA COLUBRINA (LEGUMINOSAE), A NEOTROPICAL TREE SPECIES

Autor(es)

Feres, Juliana Massimino ^{[1, 2]} ; Monteiro, Mariza ^[3] ; Zucchi, Maria I. ^[4] ; Pinheiro, Jose B. ^[3] ; Mestriner, Moacyr A. ^[1] ; Alzate-Marin, Ana Lilia ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Genet, Fac Med Ribeirao Preto, BR-14049900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Program Posgrad Genet, Fac Med Ribeirao Preto, BR-14049900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, BR-13400970 Sao Paulo - Brazil

^[4] Agencia Paulista Tecnol Agronegocios, BR-13400970 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AMERICAN JOURNAL OF BOTANY; v. 99, n. 4, p. E154-E156, APR 2012.
• Citações Web of Science: 4

Resumo

Premise of the study: We developed and characterized nuclear microsatellite markers for Anadenanthera colubrina, a tropical tree species widely distributed in South America. Methods and Results: Leaf samples of mature A. colubrina trees, popularly called ``angico,{''} were collected from an area that is greatly impacted by agricultural practices in the region of Ribeirao Preto in Sao Paulo State in southeastern Brazil. Twenty simple sequence repeat (SSR) markers were developed, 14 of which had polymorphic loci. A total of 96 alleles were detected with an average of 6.86 alleles per polymorphic locus. The expected heterozygosity, calculated at polymorphic loci, ranged from 0.18 to 0.83. Finally, we demonstrated that 18 loci were cross-amplified in A. peregrina. Conclusions: A total of 14 polymorphic markers suggest a high potential for genetic diversity, gene flow, and mating system analyses in A. colubrina. (AU)

• Processo FAPESP: 09/14200-6 - Diversidade genética, fluxo gênico e sistema de cruzamento de Anadenathera colubrina (Vell.) Brenan e Anadenanthera peregrina (L.) Speg: duas espécies que ocorrem em alta densidade em pequenas populações no interior do estado de São Paulo
• Beneficiário: Juliana Massimino Feres
• Modalidade de apoio: Bolsas no Brasil - Doutorado