Changes of Large Molecular Weight Hyaluronan and Versican in the Mouse Pubic Symphysis Through Pregnancy

Autor(es)

Rosa, Renata Giardini ^{[1, 2, 3]} ; Akgul, Yucel ^{[1, 2]} ; Joazeiro, Paulo Pinto ^[3] ; Mahendroo, Mala ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Texas SW Med Ctr Dallas, Dept Obstet & Gynecol, Dallas, TX 75390 - USA

^[2] Univ Texas SW Med Ctr Dallas, Cecil H & Ida Green Ctr Reprod Biol Sci, Dallas, TX 75390 - USA

^[3] State Univ Campinas UNICAMP, Dept Histol & Embryol, Inst Biol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOLOGY OF REPRODUCTION; v. 86, n. 2 FEB 2012.
• Citações Web of Science: 8

Resumo

During pregnancy, the mouse pubic symphysis undergoes expansion and remodeling resulting in formation of a flexible and elastic interpubic ligament allowing passage of a term fetus. In the current study, we sought to identify and characterize components of the extracellular matrix that likely play an important role in elongation and flexibility of the interpubic ligament during parturition. Mouse pubic symphyses and interpubic ligaments collected at time points during pregnancy and postpartum were utilized to evaluate collagen type, collagen content, processing and solubility, matricellular protein, and proteoglycan expression and quantitative assessment of all glycosaminoglycans. These studies revealed increased gene expression for hyaluronan synthase 1, hyaluronan synthase 2, and versican on Gestation Day 18 as well as a decline in protein expression for the versican-degrading protease a disintegrin-like and metalloprotease with thrombospondin type 1 (ADAMTS1) motif. These findings suggest that the primary mediators of increased elongation and flexibility of the interpubic ligament at term result from increased synthesis and reduced metabolism of viscoelasticity-promoting molecules such as high molecular weight hyaluronan and versican. (AU)

• Processo FAPESP: 05/51844-8 - Detecção de metaloproteinases da família das gelatinases e de seus inibidores no ligamento interpúbico do camundongo, durante a prenhez
• Beneficiário: Renata Giardini Rosa
• Modalidade de apoio: Bolsas no Brasil - Mestrado