Self-Assembly of Amphiphilic Glycoconjugates into Lectin-Adhesive Nanoparticles

Autor(es)

Dal Bo, Alexandre G. ^{[1, 2]} ; Soldi, Valdir ^[1] ; Giacomelli, Fernando C. ^[3] ; Travelet, Christophe ^[2] ; Jean, Bruno ^[2] ; Pignot-Paintrand, Isabelle ^[2] ; Borsali, Redouane ^[2] ; Fort, Sebastien ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Santa Catarina, Dept Quim, BR-88040900 Florianopolis, SC - Brazil

^[2] Univ Grenoble 1, Ctr Rech Macromol Vegetales CERMAV CNRS, F-38041 Grenoble 9 - France

^[3] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210170 Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Langmuir; v. 28, n. 2, p. 1418-1426, JAN 17 2012.
• Citações Web of Science: 25

Resumo

This work describes the synthesis and self-assembly of carbohydrate-clicked rod-coil amphiphilic systems. Copper-catalyzed Huisgen cycloaddition was efficiently employed to functionalize the hydrophilic extremity of PEG-b-tetra(p-phenylene) conjugates by lactose and N-acetyl-glucosamine ligands. The resulting amphiphilic systems spontaneously self-assembled into nanoparticles when dissolved in aqueous media, as evidenced by dynamic light scattering (DLS), transmission electron microscopy (TEM), and small-angle X-ray scattering (SAXS). The formation of highly monodisperse micelles having a mean diameter of 10 nm was observed for systems containing a PEG 900 core, and a decrease in the hydrophilic moiety (PEG 600) led to the formation of vesicles with a broader size distribution. The presence of carbohydrate residues on the surfaces of the micelles and their ability to establish specific interactions with wheat germ agglutinin (WGA) and peanut agglutinin (PNA) were further highlighted by light-scattering measurements, thus confirming the attractive applications of such sugar micelles in biosensor devices. (AU)

• Processo FAPESP: 10/06348-0 - Desenvolvimento de sistemas poliméricos supramoleculares versáteis para aplicação na liberação controlada de agentes antitumorais
• Beneficiário: Fernando Carlos Giacomelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular