Indirect determination of sulfite using a polyphenol oxidase biosensor based on a glassy carbon electrode modified with multi-walled carbon nanotubes and gold nanoparticles within a poly(allylamine hydrochloride) film

Autor(es)

Sartori, Elen Romao ^[1] ; Vicentini, Fernando Campanha ^[1] ; Fatibello-Filho, Orlando ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Quim, BR-13560970 Sao Carlos, SP - Brazil

^[2] Inst Nacl Ciencias & Tecnol Bioanalit, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Talanta; v. 87, p. 235-242, DEC 15 2011.
• Citações Web of Science: 28

Resumo

The modification of a glassy carbon electrode with multi-walled carbon nanotubes and gold nanopartides within a poly(allylamine hydrochloride) film for the development of a biosensor is proposed. This approach provides an efficient method used to immobilize polyphenol oxidase (PPO) obtained from the crude extract of sweet potato (Ipomoea batatas (L.) Lam.). The principle of the analytical method is based on the inhibitory effect of sulfite on the activity of PPO, in the reduction reaction of o-quinone to catechol and/or the reaction of o-quinone with sulfite. Under the optimum experimental conditions using the differential pulse voltammetry technique, the analytical curve obtained was linear in the concentration of sulfite in the range from 0.5 to 22 mu mol L(-1) with a detection limit of 0.4 mu mol L(-1). The biosensor was applied for the determination of sulfite in white and red wine samples with results in close agreement with those results obtained using a reference iodometric method (at a 95% confidence level). (C) 2011 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 09/14454-8 - Eletrodos de carbono modificados com nanotubos de carbono e/ou nanopartículas metálicas em filmes de quitosana para a determinação de compostos de enxofre em derivados de petróleo e alimentos
• Beneficiário: Elen Julciléia Romão Sartori
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado