Bone marrow cells of swine: Collection and separation

Autor(es)

Branco, Erika ^[1] ; Cabral, Rosa ^[1] ; Gomes, Bruno Duarte ^[2] ; Kfoury, Jr., Jose Roberto ^[3] ; Miglino, Maria Angelica ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Rural Amazonia, Fed Rural Univ Amazonia, Morphol Res Lab Anim LaPMA, Fac Vet Med, BR-66077901 Belem, Para - Brazil

^[2] Fed Univ Para UFPA, Inst Biol Sci, BR-66075110 Belem, Para - Brazil

^[3] Univ Sao Paulo, Dept Surg, Sector Anat Domest Anim & Wild, Fac Vet Med & Anim Sci, BR-05508270 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROSCOPY RESEARCH AND TECHNIQUE; v. 75, n. 7, p. 917-920, JUL 2012.
• Citações Web of Science: 3

Resumo

Bone marrow is a source of stem cells for greater and easier access, which is widely studied as a provider of hematopoietic and mesenchymal cells for various purposes, mainly therapeutic by the advances in research involving cell therapy. The swine is an animal species commonly used in the pursuit of development of experimental models. Thus, this study aimed to standardize protocol for collection and separation of bone marrow in swines, since this species is widely used as experimental models for various diseases. Twelve animals were used, which underwent bone marrow puncture with access from the iliac crest and cell separation by density gradient followed by a viability test with an average of 98% of viable cells. Given our results, we can ensure the swine as an excellent model for obtaining and isolation of mononuclear cells from bone marrow, stimulating several studies addressing the field of cell therapy. Microsc. Res. Tech., 2012. (C) 2012 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 05/50081-0 - Transplante autólogo de células mononucleares de medula óssea em suínos portadores de doença cardíaca isquemia tratados via transpericárdica e doença pulmonar intersticial tratados por via sistêmica e doenças pulmonares intersticiais
• Beneficiário: Maria Angelica Miglino
• Modalidade de apoio: Auxílio à Pesquisa - Regular