Tissue expression patterns of Schistosoma mansoni Venom Allergen-Like proteins 6 and 7

Autor(es)

Rofatto, Henrique K. ^[1] ; Parker-Manuel, Sophia J. ^[2] ; Barbosa, Tereza C. ^[1] ; Tararam, Cibele A. ^[1] ; Wilson, R. Alan ^[2] ; Leite, Luciana C. C. ^[1] ; Farias, Leonardo P. ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Ctr Biotecnol, BR-05503900 Sao Paulo - Brazil

^[2] Univ York, Dept Biol, Ctr Infect & Immun, York YO10 5DD, N Yorkshire - England

• Tipo de documento: Artigo Científico
• Fonte: International Journal for Parasitology; v. 42, n. 7, p. 613-620, JUN 2012.
• Citações Web of Science: 29

Resumo

The Schistosoma mansoni Venom Allergen-Like proteins (SmVALs) are members of the SCP/TAPS (Sperm-Coating Protein/Tpx-1/Ag5/PR-1/Sc7) protein superfamily, which may be important in host-pathogen interactions. Whole mount in situ hybridisation demonstrated a distinct expression pattern in oral and ventral suckers of adult worms for SmVAL6 and in the oesophageal gland for SmVAL7 transcripts, respectively. Additionally, immunocytochemistry analysis corroborated SmVAL7 expression in the oesophageal gland. Analysis of protein expression across the parasite's life cycle revealed that the SmVAL6 protein is upregulated in cercariae and adult male worms. Furthermore, SmVAL6 protein was identified by mass spectrometry in tegument fractions of adult worms. Finally, we speculate on possible functions of these two SmVALs at the host-parasite interface. (C) 2012 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 00/11624-5 - Embriologia de Gastropoda: análise em microscopia de varredura laser confocal
• Beneficiário: Toshie Kawano
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários