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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

A Streamlined DNA Tool for Global Identification of Heavily Exploited Coastal Shark Species (Genus Rhizoprionodon)

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Autor(es):
Pinhal, Danillo [1] ; Shivji, Mahmood S. [2, 3] ; Nachtigall, Pedro G. [1] ; Chapman, Demian D. [4, 5] ; Martins, Cesar [6]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] UNESP Sao Paulo State Univ, Dept Genet, Sao Paulo - Brazil
[2] Nova SE Univ, Save Our Seas Shark Ctr, Dania, FL - USA
[3] Nova SE Univ, Oceanog Ctr, Guy Harvey Res Inst, Dania, FL - USA
[4] SUNY Stony Brook, Sch Marine & Atmospher Sci, Stony Brook, NY 11794 - USA
[5] SUNY Stony Brook, Inst Ocean Conservat Sci, Stony Brook, NY 11794 - USA
[6] UNESP Sao Paulo State Univ, Dept Morphol, Sao Paulo - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 7, n. 4 APR 9 2012.
Citações Web of Science: 11
Resumo

Obtaining accurate species-specific landings data is an essential step toward achieving sustainable shark fisheries. Globally distributed sharpnose sharks (genus Rhizoprionodon) exhibit life-history characteristics (rapid growth, early maturity, annual reproduction) that suggests that they could be fished in a sustainable manner assuming an investment in monitoring, assessment and careful management. However, obtaining species-specific landings data for sharpnose sharks is problematic because they are morphologically very similar to one another. Moreover, sharpnose sharks may also be confused with other small sharks (either small species or juveniles of large species) once they are processed (i.e., the head and fins are removed). Here we present a highly streamlined molecular genetics approach based on seven species-specific PCR primers in a multiplex format that can simultaneously discriminate body parts from the seven described sharpnose shark species commonly occurring in coastal fisheries worldwide. The species-specific primers are based on nucleotide sequence differences among species in the nuclear ribosomal internal transcribed spacer 2 locus (ITS2). This approach also distinguishes sharpnose sharks from a wide range of other sharks (52 species) and can therefore assist in the regulation of coastal shark fisheries around the world. (AU)

Processo FAPESP: 07/03065-5 - Estrutura genético-populacional do tubarão-martelo Sphyrna lewini (Elasmobranchii: Sphyrnidae), utilizando marcadores moleculares de microssatélites
Beneficiário:César Martins
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 07/03067-8 - Estrutura genético-populacional do tubarão-martelo Sphyrna lewini (Elasmobranchii: Sphyrnidae), utilizando marcadores moleculares de microssatélites e do DNA mitocondrial
Beneficiário:Danillo Pinhal
Linha de fomento: Bolsas no Brasil - Doutorado