Quantitative Evaluation of Acetaminophen in Oral Solutions by Dispersive Raman Spectroscopy for Quality Control

Autor(es)

Borio, Viviane G. ^[1] ; Vinha, Jr., Rubens ^[2] ; Nicolau, Renata A. ^[3] ; de Oliveira, Hueder Paulo M. ^[4] ; de Lima, Carlos J. ^[1] ; Silveira, Jr., Landulfo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Camilo Castelo Branco Unicastelo, Biomed Engn Inst, BR-12247004 Sao Jose Dos Campos - Brazil

^[2] FAAP, BR-12242020 Sao Jose Dos Campos - Brazil

^[3] Univ Vale Paraiba Univap, Inst Res & Development IP&D, BR-12244000 Sao Jose Dos Campos - Brazil

^[4] Univ Fed Pelotas UFPel, Ctr Chem Sci Pharmaceut & Food, BR-96010900 Pelotas, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SPECTROSCOPY-AN INTERNATIONAL JOURNAL; v. 27, n. 4, p. 215-228, 2012.
• Citações Web of Science: 2

Resumo

This work used dispersive Raman spectroscopy to evaluate acetaminophen in commercially available formulations as an analytical methodology for quality control in the pharmaceutical industry. Raman spectra were collected using a near-infrared dispersive Raman spectrometer (830 nm, 50 mW, 20 s exposure time) coupled to a fiber optic probe. Solutions of acetaminophen diluted in excipient (70 to 120% of the commercial concentration of 200 mg/mL) were used to develop a calibration model based on partial least squares (PLSs) applied to Raman spectra of solutions and, subsequently, obtain linearity, accuracy, precision (repeatability), and sensitivity of the method using the near-infrared spectroscopy (NIRS) as a gold standard method. This model was used to predict the acetaminophen concentration in commercial samples from different lots of acetaminophen formulations (200 mg/mL) with a PLS-prediction error of about 0.6%. Commercial medicines had PLS predicted concentrations errors below 2.5%, whereas NIRS had an error of about 3.7% compared to the label concentration. It has been demonstrated the applicability of Raman spectroscopy with fiber probe for quality control in pharmaceutical industry of commercial formulations. (AU)

• Processo FAPESP: 06/56701-3 - Estudo de processos de agregacao em moleculas com potencial aplicacao em terapia fotodinamica.
• Beneficiário: Hueder Paulo Moisés de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular