Biochemical and metabolic profiles of Trichoderma strains isolated from common bean crops in the Brazilian Cerrado, and potential antagonism against Sclerotinia sclerotiorum

Autor(es)

Cardoso Lopes, Fabyano Alvares ^[1] ; Steindorff, Andrei Stecca ^[1] ; Geraldine, Alaerson Maia ^[2] ; Brandao, Renata Silva ^[1] ; Monteiro, Valdirene Neves ^[3] ; Lobo Junior, Murillo ^[2] ; Guedes Coelho, Alexandre Siqueira ^[4] ; Ulhoa, Cirano Jose ^[1] ; Silva, Roberto Nascimento ^[5]

Afiliação do(s) autor(es)

^[1] Fed Univ Goias UFG, Dept Biochem & Mol Biol, BR-74001970 Goiania, Go - Brazil

^[2] Brazilian Agr Res Corp, Embrapa Rice & Beans, BR-75375000 Santo Antonio De Goias, Go - Brazil

^[3] Univ Estadual Goias UnUCET, Unidade Univ Ciencias Exatas & Tecnol, Univ Estadual Goias, BR-75000000 Anapolis, Go - Brazil

^[4] Fed Univ Goias UFG, Sch Agron & Food Engn, BR-74001970 Goiania, Go - Brazil

^[5] Univ Sao Paulo, Sch Med, Dept Biochem & Immunol, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FUNGAL BIOLOGY; v. 116, n. 7, p. 815-824, JUL 2012.
• Citações Web of Science: 48

Resumo

Some species of Trichoderma have successfully been used in the commercial biological control of fungal pathogens, e.g., Sclerotinia sclerotiorum, an economically important pathogen of common beans (Phaseolus vulgaris L.). The objectives of the present study were (1) to provide molecular characterization of Trichoderma strains isolated from the Brazilian Cerrado; (2) to assess the metabolic profile of each strain by means of Biolog FF Microplates; and (3) to evaluate the ability of each strain to antagonize S. sclerotiorum via the production of cell wall-degrading enzymes (CWDEs), volatile antibiotics, and dual-culture tests. Among 21 isolates, we identified 42.86 % as Trichoderma asperellum, 33.33 % as Trichoderma harzianum, 14.29 % as Trichoderma tomentosum, 4.76 % as Trichoderma koningiopsis, and 4.76 % as Trichoderma erinaceum. Trichoderma asperellum showed the highest CWDE activity. However, no species secreted a specific group of CWDEs. Trichoderma asperellum 364/01, T. asperellum 483/02, and T. asperellum 356/02 exhibited high and medium specific activities for key enzymes in the mycoparasitic process, but a low capacity for antagonism. We observed no significant correlation between CWDE and antagonism, or between metabolic profile and antagonism. The diversity of Trichoderma species, and in particular of T. harzianum, was clearly reflected in their metabolic profiles. Our findings indicate that the selection of Trichoderma candidates for biological control should be based primarily on the environmental fitness of competitive isolates and the target pathogen. (C) 2012 The British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/15683-8 - Estudos de sinalização celular e mecanismos de indução da formação de celulases pelo fungo Trichoderma reesei (Hypocrea jecorina)
• Beneficiário: Roberto do Nascimento Silva
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores