Salivary immunity in elderly individuals presented with Candida-related denture stomatitis

Autor(es)

Gasparoto, Thais H. ^[1] ; Sipert, Carla R. ^[1] ; de Oliveira, Carine E. ^[2] ; Porto, Vinicius C. ^[3] ; Santos, Carlos F. ^[1] ; Campanelli, Ana P. ^[1] ; Lara, Vanessa S.

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Biol Sci, Bauru Sch Dent, BR-17012901 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Stomatol Oral Pathol, Bauru Sch Dent, BR-17012901 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Prosthodont, Bauru Sch Dent, BR-17012901 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GERODONTOLOGY; v. 29, n. 2, p. E331-E339, JUN 2012.
• Citações Web of Science: 14

Resumo

Objectives: Elderly individuals with Candida-related denture stomatitis (DS) present with a reduced defence against Candida albicans. This study evaluated levels of antimicrobial mediators in the elderly DS saliva and salivary neutrophils' activation characteristics compared with elderly and young without DS. Methods: Salivary peroxidases (SPO) and elastase activities (ELA), nitric oxide (NO), transforming growth factor beta (TGF-beta), IL-6 and CCL3 production were determined in saliva from elderly with or without DS, and young control individuals. TLR4, CXCR1, CD11b, CD16 and CD32 expression on salivary neutrophils were evaluated. Correlations between number and apoptosis rate of salivary neutrophils, enzymatic activities and cytokine levels were determined. Results: Elderly DS individuals exhibited the lowest SPO and ELA activities. Also, the activity of both enzymes was low in elderly without DS. Although both elderly groups showed higher salivary NO and TGF-beta levels compared to young control groups, elderly DS presented the highest salivary NO, TGF-beta, IL-6 and CCL3 levels. Decreased percentages of salivary TLR4(+) and CD16(+) neutrophils were detected in both elderly groups. Although these damages could influence the establishment and persistence of DS, the highest levels of salivary IL-6 and CCL3 in elderly DS could be preventing more serious complications. (AU)

• Processo FAPESP: 05/60668-9 - Estudo da função dos neutrófilos nos mecanismos de defesa contra a estomatite por dentadura em indivíduos idosos
• Beneficiário: Thaís Helena Gasparoto
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/00306-1 - Expressão de pró-colágeno do tipo I, MIP-1alfa e SDF-1alfa por fibroblastos de gengiva e polpa dental humanas estimulados por ácido lipoteicóico de Enterococcus faecalis e lipopolissacarídeo de Escherichia coli e Porphyromonas gingivalis
• Beneficiário: Carla Renata Sipert
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 06/59612-1 - Senescência e estomatite por dentadura: avaliação quantitativa, funcional e fenotípica dos neutrófilos
• Beneficiário: Vanessa Soares Lara
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/60167-0 - Expressão de pró-colágeno tipo 1, MIP-1± alfa e SDF-1 alfa por fibroblastos da polpa humana estimulados por ácido lipoteicóico de Streptococcus mutans
• Beneficiário: Carlos Ferreira dos Santos
• Linha de fomento: Auxílio à Pesquisa - Regular