Relationship Between Expression of Voltage-Dependent Anion Channel (VDAC) Isoforms and Type of Hexokinase Binding Sites on Brain Mitochondria

Autor(es)

Poleti, Mirele Daiana ^[1] ; Tesch, Andrea Cristina ^[1] ; Crepaldi, Carla Rossini ^[1] ; Martins Ferreira Souza, Gustavo Henrique ^[2] ; Eberlin, Marcos Nogueira ^[2] ; Cesar, Marcelo de Cerqueira ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Anim Sci & Food Engn, Lab Neurosci & Prote, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Estadual Campinas, Inst Chem, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MOLECULAR NEUROSCIENCE; v. 41, n. 1, p. 48-54, MAY 2010.
• Citações Web of Science: 13

Resumo

Voltage-dependent anion channels (VDAC) are pore-forming proteins found in the outer mitochondrial membrane of eukaryotes. VDACs are known to play an essential role in cellular metabolism and in early stages of apoptosis. In mammals, three VDAC isoforms have been identified. A proteomic approach was exploited to study the expression of VDAC isoforms in rat, bovine, and chicken brain mitochondria. Given the importance of mitochondrially bound hexokinase in regulation of aerobic glycolysis in brain, we studied the possibility that differences in the relative expression of VDAC isoforms may be a factor in determining the species-dependent ratio of type A/type B hexokinase binding sites on brain mitochondria. The spots were characterized, and the signal intensities among spots were compared. VDAC1 was the most abundantly expressed of the three isoforms. Moreover the expression of VDAC1 plus VDAC2 was significantly higher in bovine than in rat brain. Chicken brain mitochondria showed the highest VDAC1 expression and the lowest of VDAC2. Bovine brain mitochondria had the highest VDAC2 levels. We concluded that the nature of hexokinase binding site is not determined by the expression of a single VDAC isoform. (AU)

• Processo FAPESP: 04/03170-5 - Aspectos moleculares do metabolismo energético em cérebro de aves, ratos e ruminantes: quantificação da expressão gênica de proteínas chave do metabolismo de glicose. Análise espécie - especifica via espectrometria de massa da interação hexoquinase - VDA
• Beneficiário: Marcelo de Cerqueira César
• Modalidade de apoio: Auxílio à Pesquisa - Temático