The Gracilariaceae Germplasm Bank of the University of So Paulo, Brazil-a DNA barcoding approach

Autor(es)

Costa, Emmanuelle S. ^[1] ; Plastino, Estela M. ^[1] ; Petti, Rosario ^[1] ; Oliveira, Eurico C. ^{[1, 2]} ; Oliveira, Mariana C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Bot, Inst Biociencias, BR-05508900 Sao Paulo - Brazil

^[2] Univ Fed Santa Catarina, Ctr Ciencias Biol, Dept Bot, Florianopolis, SC - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF APPLIED PHYCOLOGY; v. 24, n. 6, p. 1643-1653, DEC 2012.
• Citações Web of Science: 18

Resumo

The University of So Paulo Gracilariaceae Germplasm Bank has 50 strains collected mostly in Brazil, but also elsewhere in the world. This bank has been used as a source of material for research developed locally and abroad. With over 200 species, some of which have high economic value, the family Gracilariaceae has been extensively studied. Nonetheless, taxonomic problems still persist by the existence of cryptic species, phenotypic plasticity, and broad geographic distribution. In the case of algae kept in culture for long periods of time, the identification is even more problematic as a consequence of considerable morphological modification. Thus, the use of molecular markers has been shown to be an efficient tool to elucidate taxonomic issues in the group. In this work, we sequenced the 5'-end of the cox1 gene for 41 strains and the universal plastid amplicon (UPA) plastid region for 45 strains, covering all 50 strains in the bank. In addition, the rbcL for representatives of the cox1/UPA clusters was sequenced for 14 strains. The original species identification based on morphology was compared with the molecular data obtained in this work, resulting in the identification of 13 different species. Our analyses indicate that cox1 and UPA are suitable markers for the delineation of species of Gracilariales in the germplasm bank. The addition of DNA barcode tags to the samples in the Gracilariaceae germplasm bank and the molecular identification of the species will make this bank even more useful for future research as the species can be easily traced and confirmed. (AU)

• Processo FAPESP: 07/51270-7 - Diversidade das microalgas vermelhas (Rhodophyta) do estado de São Paulo baseada em barcoding, morfologia e distribuição geográfica (RHODO-SP)
• Beneficiário: Mariana Cabral de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático