3-Hydroxypropionic Acid as an Antibacterial Agent from Endophytic Fungi Diaporthe phaseolorum

Autor(es)

Sebastianes, Fernanda L. S. ^[1] ; Cabedo, Nuria ^[2] ; El Aouad, Noureddine ^[3] ; Valente, Angela M. M. P. ^[4] ; Lacava, Paulo T. ^[5] ; Azevedo, Joao L. ^[1] ; Pizzirani-Kleiner, Aline A. ^[1] ; Cortes, Diego ^[6]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, BR-13400970 Piracicaba, SP - Brazil

^[2] Univ Politecn Valencia, Ctr Ecol Quim Agr, Inst Agroforestal Mediterraneo, Valencia 46022 - Spain

^[3] Fdn MEDINA, Ctr Excelencia Invest Medicamentos Innovadores An, Ctr Desarrollo Farmaceut & Alimentario Parque Tec, Granada 18100 - Spain

^[4] Embrapa Meio Ambiente CNPMA, Lab Microbiol Ambiental, BR-13820000 Jaguariuna, SP - Brazil

^[5] Univ Fed Alfenas, Inst Nat Sci, BR-37130000 Alfenas, MG - Brazil

^[6] Univ Valencia, Fac Farm, Dept Farmacol, Valencia 46100 - Spain

• Tipo de documento: Artigo Científico
• Fonte: Current Microbiology; v. 65, n. 5, p. 622-632, NOV 2012.
• Citações Web of Science: 25

Resumo

Endophytic fungi are considered a rich source of active compounds resulting from their secondary metabolism. Fungi from marine environment grow in a habitat with unique conditions that can contribute to the activation of metabolic pathways of synthesis of different unknown molecules. The production of these compounds may support the adaptation and survival of the fungi in the marine ecosystem. Mangroves are ecosystems situated between land and sea. They are frequently found in tropical and subtropical areas and enclose approximately 18.1 million hectares of the planet. The great biodiversity found in these ecosystems shows the importance of researching them, including studies regarding new compounds derived from the endophytic fungi that inhabit these ecosystems. 3-hydroxypropionic acid (3-HPA) has been isolated from the mangrove endophytic fungus Diaporthe phaseolorum, which was obtained from branches of Laguncularia racemosa. The structure of this compound was elucidated by spectroscopic methods, mainly 1D and 2D NMR. In bioassays, 3-HPA showed antimicrobial activities against both Staphylococcus aureus and Salmonella typhi. The structure of this antibiotic was modified by the chemical reaction of Fischer-Speier esterification to evaluate the biologic activity of its chemical analog. The esterified product, 3-hydroxypropanoic ethyl ester, did not exhibit antibiotic activity, suggesting that the free carboxylic acid group is important to the pharmacological activity. The antibiotic-producing strain was identified with internal transcribed spacer sequence data. To the best of our knowledge, this is the first report of antibacterial activity by 3-HPA against the growth of medically important pathogens. (AU)

• Processo FAPESP: 04/13910-6 - Biodiversidade e atividades funcionais de microrganismos de manguezais do estado de São Paulo
• Beneficiário: Itamar Soares de Melo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 06/57060-1 - Análise da comunidade bacteriana associada a manguezais do estado de São Paulo com interesse biotecnológico e agrícola
• Beneficiário: Paulo Teixeira Lacava
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado