The Effect of Supra- and Subphysiologic Testosterone Levels on Ligature-Induced Bone Loss in Rats - A Radiographic and Histologic Pilot Study

Autor(es)

Steffens, Joao P. ^[1] ; Coimbra, Leila S. ^[1] ; Ramalho-Lucas, Pablo D. ^[1] ; Rossa, Jr., Carlos ^[2] ; Spolidorio, Luis C. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Sch Dent Araraquara, Dept Physiol & Pathol, Sao Paulo - Brazil

^[2] Sao Paulo State Univ, Dept Diag & Surg, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Periodontology; v. 83, n. 11, p. 1432-1439, NOV 2012.
• Citações Web of Science: 10

Resumo

Background: Testosterone is the primary male sexual hormone, and varying concentrations of the hormone mediated by physiologic, pathologic, or pharmacologic mechanisms may induce large variations in the body. Data regarding the general role of testosterone in mediating inflammation are still inconclusive. Therefore, the purpose of this study is to assess the consequences of supra- and subphysiologic levels of testosterone on ligature-induced bone loss in rats. Methods: Three male adult Holtzman rats were used to observe the course of serum testosterone concentration following orchiectomy (Ocx) and testosterone injections. Another 60 rats were randomly assigned to the following groups: 1) sham-operation controls (n = 10); 2) sham-operation and ligature-induced bone loss (n = 10); 3) orchiectomy without ligature (Ocx; n = 10); 4) Ocx and ligature (n = 10); 5) Ocx plus 250 mg/kg body weight intramuscular testosterone esters injection without ligature (Ocx+T; n = 10); and 6) Ocx, T, and ligature (n = 10). The ligatures were placed 30 days post-orchiectomy (or sham-operation) and maintained for 15 days. Thereafter, the rats were sacrificed, and their hemimandibles were used for radiographic evaluation of bone loss along with histologic and histometric analyses of gingival tissue. Results: The results indicated a significant increase in bone loss in the Ocx and Ocx+T groups in the presence and absence of inflammation, respectively. In addition, the Ocx and Ocx+T groups presented increased gingival area accompanying ligature-induced bone loss. Conclusions: Both sub- and supraphysiologic testosterone levels may influence bone metabolism, but only subphysiologic levels significantly increase ligature-induced bone loss. Moreover, testosterone has a regulatory effect on the gingival area. J Periodontol 2012;83:1432-1439. (AU)

• Processo FAPESP: 10/12021-4 - Efeito da redução dos níveis de testosterona na resposta imunoinflamatória associada à indução de doença periodontal e artrite reumatóide em ratos adultos orquiectomizados
• Beneficiário: Luis Carlos Spolidorio
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/09658-0 - Efeito da redução dos níveis de testosterona na resposta imunoinflamatória associada à indução de doença periodontal e artrite reumatoide em ratos adultos orquiectomizados
• Beneficiário: João Paulo Steffens
• Modalidade de apoio: Bolsas no Brasil - Doutorado