Cu(GlyGlyHis) effects on MCF7 cells: Copper uptake, reactive oxygen species generation and membrane topography changes

Autor(es)

Matias, Andreza C. ^[1] ; dos Santos, Nathalia Villa ^[1] ; Chelegao, Rodrigo ^[1] ; Nomura, Cassiana S. ^[2] ; Fiorito, Pablo A. ^[1] ; Cerchiaro, Giselle ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ ABC UFABC, Ctr Nat Sci & Humanities, BR-09210170 Santo Andre, SP - Brazil

^[2] Univ Sao Paulo, Inst Chem, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Inorganic Biochemistry; v. 116, p. 172-179, NOV 2012.
• Citações Web of Science: 9

Resumo

Elevated levels of copper have been detected in various types of human cancer cells, such as breast cancer cells, and a number of mechanisms have been proposed to explain the action and influence of copper on tumor progress. In this work, we found that stimulating the proliferation of mammary epithelial MCF7 cells with the high-redox-potential copper complex Cu (GlyGlyHis) is associated with the copper-induced intracellular generation of reactive oxygen species (ROS) that induces lipid peroxidation and causes increased roughness of external cell membranes, which leads to the formation of larger cell domes. The results presented herein provide new insights into the molecular link between copper and the proliferation of breast cancer cells and, consequently, into the mechanism by which changes in redox balance and ROS accumulation regulates cell membrane roughness. (C) 2012 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 07/50765-2 - Estudo de processos oxidativos e radicalares envolvendo o cobre no meio biológico: uma investigação de mecanismos moleculares da atividade oxidante de complexos e proteínas contendo cobre
• Beneficiário: Giselle Cerchiaro
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores