| Texto completo | |
| Autor(es): |
Trindade-Silva, Amaro E.
[1, 2]
;
Rua, Cintia P. J.
[2]
;
Andrade, Bruno G. N.
[3]
;
Paulo Vicente, Ana Carolina
[3]
;
Silva, Genivaldo G. Z.
[4]
;
Berlinck, Roberto G. S.
[1]
;
Thompson, Fabiano L.
[2]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Quim Sao Carlos, Sao Carlos, SP - Brazil
[2] Univ Fed Rio de Janeiro, Inst Biol, Rio De Janeiro - Brazil
[3] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Rio De Janeiro - Brazil
[4] San Diego State Univ, Dept Comp Sci, San Diego, CA 92182 - USA
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Applied and Environmental Microbiology; v. 79, n. 5, p. 1598-1605, MAR 2013. |
| Citações Web of Science: | 14 |
| Resumo | |
Microbes associated with marine sponges are considered important producers of bioactive, structurally unique polyketides. The synthesis of such secondary metabolites involves type I polyketide synthases (PKSs), which are enzymes that reach a maximum complexity degree in bacteria. The Haplosclerida sponge Arenosclera brasiliensis hosts a complex microbiota and is the source of arenosclerins, alkaloids with cytotoxic and antibacterial activity. In the present investigation, we performed high-throughput sequencing of the ketosynthase (KS) amplicon to investigate the diversity of PKS genes present in the metagenome of A. brasiliensis. Almost 4,000 ketosynthase reads were recovered, with about 90% annotated automatically as bacterial. A total of 235 bacterial KS contigs was rigorously assembled from this sequence pool and submitted to phylogenetic analysis. A great diversity of six type I PKS groups has been consistently detected in our phylogenetic reconstructions, including a novel and A. brasiliensis-exclusive group. Our study is the first to reveal the diversity of type I PKS genes in A. brasiliensis as well as the potential of its microbiome to serve as a source of new polyketides. (AU) | |
| Processo FAPESP: | 09/11612-1 - Caracterização do grupamento gênico de biossíntese de Arenosclerinas, alcaloides alquilpiperidínicos isolados da esponja Arenosclera brasiliensis |
| Beneficiário: | Amaro Emiliano Trindade Silva |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 10/50190-2 - Investigação do potencial biotecnológico e metabólico de organismos marinhos para processos de biorremediação e produção de substâncias com atividades anti-virais, anti-leishmania e anti-inflamatória (Biodiversidade Marinha) |
| Beneficiário: | Roberto Gomes de Souza Berlinck |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOTA - Temático |