Use of reverse transcriptase polymerase chain reaction (RT-PCR) in molecular screening of Newcastle disease virus in poultry and free-living bird populations

Autor(es)

Torres Carrasco, Adriano de Oliveira ^[1] ; Martins Rodrigues, Juliana Nogueira ^[2] ; Seki, Meire Christina ^[3] ; de Moraes, Fabricio Edgar ^[4] ; Silva, Jaqueline Raymondi ^[3] ; Durigon, Edison Luis ^[2] ; Pinto, Aramis Augusto ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Centro Oeste, UNICENTRO, Dept Med Vet, BR-85040080 Guarapuava, Parana - Brazil

^[2] Univ Sao Paulo, Dept Microbiol, BR-05508900 Sao Paulo - Brazil

^[3] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Patol Vet, BR-14884900 Sao Paulo - Brazil

^[4] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Tecnol, BR-14884900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TROPICAL ANIMAL HEALTH AND PRODUCTION; v. 45, n. 2, p. 569-576, FEB 2013.
• Citações Web of Science: 2

Resumo

The aim of this study was to evaluate a simple molecular method of reverse transcriptase polymerase chain reaction (RT-PCR) to differentiate Newcastle disease virus strains according to their pathogenicity, in order to use it in molecular screening of Newcastle disease virus in poultry and free-living bird populations. Specific primers were developed to differentiate LaSota-LS-(vaccine strain) and Sao Joao do Meriti-SJM-strain (highly pathogenic strain). Chickens and pigeons were experimentally vaccinated/infected for an in vivo study to determine virus shedding in feces. Validation of sensitivity and specificity of the primers (SJM and LS) by experimental models used in the present study and results obtained in the molecular analysis of the primers by BLAST made it possible to generalize results. The development of primers that differentiate the level of pathogenicity of NDV stains is very important, mainly in countries where real-time RT-PCR is still not used as a routine test. These primers were able to determine the presence of the agent and to differentiate it according to its pathogenicity. (AU)

• Processo FAPESP: 04/13868-0 - Estudo da infectividade, transmissibilidade, estado de portador (reservatorio) e da resposta imune humoral de pombos (columba livia) submetidos a infeccao experimental frente ao virus da doenca de ...
• Beneficiário: Adriano de Oliveira Torres Carrasco
• Modalidade de apoio: Bolsas no Brasil - Doutorado