| Texto completo | |
| Autor(es): |
Maranhao, Fernanda C. A.
[1]
;
Paiao, Fernanda G.
[1]
;
Fachin, Ana Lucia
[1]
;
Martinez-Rossi, Nilce M.
[1]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14049900 Sao Paulo - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Medical Microbiology; v. 58, n. 2, p. 163-168, Feb. 2009. |
| Área do conhecimento: | Ciências Biológicas - Genética |
| Citações Web of Science: | 24 |
| Assunto(s): | Fungos Trichophyton Arthrodermataceae |
| Resumo | |
Trichophyton rubrum is a dermatophyte responsible for the majority of human superficial mycoses. The functional expression of proteins important for the initial step and the maintenance of the infection process were identified previously in T. rubrum by subtraction suppression hybridization after growth in the presence of keratin. In this study, sequences similar to genes encoding the multidrug-resistance ATP-binding cassette (ABC) transporter, copper ATPase, the major facilitator superfamily and a permease were isolated, and used in Northern blots to monitor the expression of the genes, which were upregulated in the presence of keratin. A sequence identical to the TruMDR2 gene, encoding an ABC transporter in T rubrum, was isolated in these experiments, and examination of a T rubrum Delta TruMDR2 mutant showed a reduction in infecting activity, characterized by low growth on human nails compared with the wild-type strain. The high expression levels of transporter genes by T. rubrum in mimetic infection and the reduction in virulence of the Delta TruMDR2 mutant in a disease model in vitro suggest that transporters are involved in T. rubrum pathogenicity. (AU) | |
| Processo FAPESP: | 08/58634-7 - Genômica funcional e comparativa em fungos |
| Beneficiário: | Nilce Maria Martinez-Rossi |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |