Satellite DNA sites in four species of the genus Astyanax (Teleostei, Characiformes)

Autor(es)

Karine Frehner Kavalco ^[1] ; Rubens Pazza ^[2] ; Luiz Antonio Carlos Bertollo ^[3] ; Orlando Moreira-Filho ^[4]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Instituto de Biociências. Departamento de Genética e Biologia Evolutiva - Brasil

^[2] Centro Universitário Nove de Julho. Grupo de Pesquisas em Biodiversidade e Conservação - Brasil

^[3] Universidade Federal de São Carlos. Departamento de Genética e Evolução - Brasil

^[4] Universidade Federal de São Carlos. Departamento de Genética e Evolução - Brasil

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 30, n. 3, p. 529-535, 2007-00-00.

Resumo

Cytogenetic data about satellite DNA distribution in four Astyanax species (Characidae) from the Paraitinga river, Paraíba do Sul river basin, Brazil, are presented. In order to characterize the constitutive heterochromatin, C-banding, chromomycin A3 and DAPI fluorescence staining, as well as fluorescence in situ hybridization (FISH) with the satellite DNA As-51 probe were performed. A. scabripinnis and A. parahybae presented 2n = 50 and 2n = 48 chromosomes, respectively. The heterochromatin was located in the pericentromeric and terminal regions of many chromosomes, corresponding to GC-positive regions and to the As-51 satellite DNA in terminal regions. A. intermedius and A. giton, both with 2n = 50 chromosomes, showed little heterochromatin, mostly restricted to the terminal and pericentromeric regions of a few chromosomes. No GC-positive regions, neither any correspondence between the scarce heterochromatin of these species and the As-51 satellite DNA was observed. AT-positive blocks were not detected in any of the species studied. Based on these and other available data, the hypothesis that Astyanax represents a polyphyletic group is discussed. (AU)

• Processo FAPESP: 01/00713-0 - Análise da biodiversidade de riachos, citogenética comparativa e taxonomia de ictiofaunas isoladas por um divisor de águas
• Beneficiário: Orlando Moreira Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular