A recombination point is conserved in the mitochondrial genome of higher plant species and located downstream from the cox2 pseudogene in Solanum tuberosum L.

Autor(es)

Susely F.S. Tada ^[1] ; Anete P. Souza ^[2]

Afiliação do(s) autor(es)

^[1] Universidade Estadual de Campinas. Centro de Biologia Molecular e Engenharia Genética - Brasil

^[2] Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Genética e Evolução - Brasil

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 29, n. 1, p. 83-89, 2006-00-00.

Resumo

The potato (Solanum tuberosum L.) mitochondrial cox3/sdh4/pseudo-cox2 gene cluster has previously been identified by heterologous hybridization using a Marchantia polymorpha sdh4 probe. In our present study we used Southern blotting using sdh4 and cox2 probes to show that the sdh4 and cox2 genes are clustered in the mitochondria of potato, soybean and pea. Northern blotting revealed cotranscription of sdh4 and cox2 in potato but not in cauliflower, indicating that these genes are not clustered in cauliflower. A putative recombination point was detected downstream of the cox2 pseudogene (pseudo-cox2) in potato mitochondrial DNA (mtDNA). This sequence corresponds to a 32 bp sequence which appears to be well-conserved and is adjacent to the terminals of some mitochondrial genes in Citrullus lanatus, Beta vulgaris and Arabidopsis thaliana and is probably involved in the genic rearrangements. It is possible the potato mtDNA pseudo-cox2 gene was generated by recombination during evolution in the same way as that of several other mitochondrial genes and remains as an inactive partial copy of the functional cox2 which was also detected in potato mtDNA. (AU)

• Processo FAPESP: 96/03520-8 - Estudo da estrutura e expressão de novos genes mitocondriais em plantas superiores
• Beneficiário: Anete Pereira de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 98/12723-5 - Estudo da estrutura e expressão de um novo gene mitocondrial em Solanum tuberosum L.
• Beneficiário: Susely Ferraz de Siqueira Tada
• Modalidade de apoio: Bolsas no Brasil - Doutorado