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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Supra-physiological doses of testosterone affect membrane oxidation of human neutrophils monitored by the fluorescent probe C-11-BODIPY581/591

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Autor(es):
de Souza-Junior, Tacito Pessoa [1] ; Yamada, Andre K. [2] ; Simao, Roberto [3] ; Polotow, Tatiana G. [4] ; Curi, Rui [5] ; Pope, Zachary [6] ; Willardson, Jeffrey M. [6] ; Barros, Marcelo P. [4]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Parana Fed Univ UFPR, Dept Phys Educ, Curitiba, Parana - Brazil
[2] Univ Fed Sao Carlos, Dept Physiol Sci, Sao Paulo - Brazil
[3] Univ Fed Rio de Janeiro, Rio De Janeiro - Brazil
[4] Univ Cruzeiro Sul, Inst Phys Act & Sports Sci ICAFE, BR-01506000 Sao Paulo - Brazil
[5] Univ Sao Paulo, Dept Physiol & Biophys, Sao Paulo - Brazil
[6] Eastern Illinois Univ, Dept Kinesiol & Sports Studies, Charleston, IL 61920 - USA
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY; v. 113, n. 5, p. 1241-1248, MAY 2013.
Citações Web of Science: 4
Resumo

The purpose of this study was to determine the effects of supra-physiological doses of testosterone (TES) on membrane oxidation of activated human neutrophils in vitro using an innovative and sensitive technique: the real-time detection with the fluorescence probe C-11-BODIPY581/591. Methodological controls were performed with the lipid-soluble and powerful antioxidant astaxanthin at different neutrophil density cultures. Neutrophils from nine healthy young men (23.4 +/- A 2.5 years, 174.4 +/- A 7.0 cm height, and 78.3 +/- A 7.0 kg weight) were isolated and treated with 0.1 or 10 mu M TES for 24 h and subsequently labeled with the free radical-sensitive probe C-11-BODIPY581/591 for monitoring membrane oxidation after neutrophil activation with phorbol-12-myristate-13-acetate (PMA). First-order exponential decay kinetic indicated that both 0.1 and 10 mu M TES severely increased baseline membrane oxidation in non-activated human neutrophils (compared to control). However, similar kinetics of membrane oxidation were observed in control and 0.1 mu M TES-treated neutrophils after PMA activation, whereas chemical activation did not alter the baseline higher rates of membrane oxidation in 10 mu M TES-treated neutrophils. The data presented here support the hypothesis that TES exerts distinct effects on the membrane oxidation of human neutrophils, depending on its dose (here, 10(2) to 10(4)-fold higher than physiological levels in men) and on PMA activation of the oxidative burst. Furthermore, this paper also presents an innovative application of the free radical-sensitive probe C-11-BODIPY581/591 for monitoring (auto-induced) membrane oxidation as an important parameter of viability and, thus, responsiveness of immune cells in inflammatory processes. (AU)

Processo FAPESP: 09/12342-8 - Ação do carotenóide astaxantina na regulação do equilíbrio redox no músculo esquelético de ratos sob atividade física exaustiva (natação): projeto AstaSwim
Beneficiário:Andréa Ramalho Mihaliuc
Modalidade de apoio: Bolsas no Brasil - Mestrado