Biochemical and Electrophysiological Characterization of Two Sea Anemone Type 1 Potassium Toxins from a Geographically Distant Population of Bunodosoma caissarum

Autor(es)

Orts, Diego J. B. ^{[1, 2]} ; Peigneur, Steve ^[3] ; Madio, Bruno ^[1] ; Cassoli, Juliana S. ^[4] ; Montandon, Gabriela G. ^[4] ; Pimenta, Adriano M. C. ^[4] ; Bicudo, Jose E. P. W. ^[1] ; Freitas, Jose C. ^[1] ; Zaharenko, Andre J. ^[5] ; Tytgat, Jan ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biosci, Dept Physiol, BR-05508090 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Ctr Marine Biol, BR-11600000 Sao Sebastiao, SP - Brazil

^[3] Univ Leuven KU Leuven, Toxicol Lab, B-3000 Louvain - Belgium

^[4] Univ Fed Minas Gerais, Inst Biol Sci, Lab Venoms & Anim Toxins, BR-31270901 Belo Horizonte, MG - Brazil

^[5] Inst Butantan, Genet Lab, BR-05503900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MARINE DRUGS; v. 11, n. 3, p. 655-679, MAR 2013.
• Citações Web of Science: 17

Resumo

Sea anemone (Cnidaria, Anthozoa) venom is an important source of bioactive compounds used as tools to study the pharmacology and structure-function of voltage-gated K+ channels (K-V). These neurotoxins can be divided into four different types, according to their structure and mode of action. In this work, for the first time, two toxins were purified from the venom of Bunodosoma caissarum population from Saint Peter and Saint Paul Archipelago, Brazil. Sequence alignment and phylogenetic analysis reveals that BcsTx1 and BcsTx2 are the newest members of the sea anemone type 1 potassium channel toxins. Their functional characterization was performed by means of a wide electrophysiological screening on 12 different subtypes of K-V channels (K(V)1.1-K(V)1.6; K(V)2.1; K(V)3.1; K(V)4.2; K(V)4.3; hERG and Shaker IR). BcsTx1 shows a high affinity for rKv1.2 over rKv1.6, hKv1.3, Shaker IR and rKv1.1, while Bcstx2 potently blocked rKv1.6 over hKv1.3, rKv1.1, Shaker IR and rKv1.2. Furthermore, we also report for the first time a venom composition and biological activity comparison between two geographically distant populations of sea anemones. (AU)

• Processo FAPESP: 07/56525-3 - Construção de uma biblioteca de cDNA dos tentáculos da anêmona do mar Bunodosoma cangicum e clonagem de neurotoxinas
• Beneficiário: Andre Junqueira Zaharenko
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/21031-6 - Análise do mecanismo de ação envolvido na seletividade da atividade do peptídeo Abe III 1.3 em canais voltagem-dependentes de K+
• Beneficiário: Diego Jose Orts y Belato
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
• Processo FAPESP: 09/07128-7 - Neurotoxinas de anêmonas do mar como ferramentas para o estudo da fisiologia de canais voltagem - dependentes de potássio
• Beneficiário: Diego Jose Orts y Belato
• Modalidade de apoio: Bolsas no Brasil - Mestrado