Low-Level Laser Therapy and Sodium Diclofenac in Acute Inflammatory Response Induced by Skeletal Muscle Trauma: Effects in Muscle Morphology and mRNA Gene Expression of Inflammatory Markers

Autor(es):Mostrar menos -

de Almeida, Patricia ^[1] ; Brandao Lopes-Martins, Rodrigo Alvaro ^{[1, 2, 3]} ; Tomazoni, Shaiane Silva ^[3] ; Albuquerque-Pontes, Gianna Moes ^[2] ; Santos, Larissa Aline ^[1] ; Vanin, Adriane Aver ^[1] ; Frigo, Lucio ^[4] ; Vieira, Rodolfo P. ^{[1, 2]} ; Albertini, Regiane ^{[1, 2]} ; Camillo de Carvalho, Paulo de Tarso ^{[1, 2]} ; Pinto Leal-Junior, Ernesto Cesar ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Nove Julho Univ UNINOVE, Post Grad Program Rehabil Sci, Sao Paulo - Brazil

^[2] Nove Julho Univ UNINOVE, Post Grad Program Biophoton Appl Hlth Sci, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Pharmacol, Inst Biomed Sci, Lab Pharmacol & Expt Therapeut, Sao Paulo - Brazil

^[4] Univ Cruzeiro Sul, Biol Sci & Hlth Ctr, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photochemistry and Photobiology; v. 89, n. 2, p. 501-507, MAR-APR 2013.
• Citações Web of Science: 25

Resumo

Pharmacological therapy is widely used in the treatment of muscle injuries. On the other hand, low-level laser therapy (LLLT) arises as a promising nonpharmacological treatment. The aim of this study was to analyze the effects of sodium diclofenac (topical application) and LLLT on morphological aspects and gene expression of biochemical inflammatory markers. We performed a single trauma in tibialis anterior muscle of rats. After 1h, animals were treated with sodium diclofenac (11.6mg g-1 of solution) or LLLT (810nm; continuous mode; 100mW; 3.57Wcm2; 1, 3 or 9J; 10, 30 or 90s). Histological analysis and quantification of gene expression (real-time polymerase chain reactionRT-PCR) of cyclooxygenase 1 and 2 (COX-1 and COX-2) and tumor necrosis factor-alpha (TNF-) were performed at 6, 12 and 24h after trauma. LLLT with all doses improved morphological aspects of muscle tissue, showing better results than injury and diclofenac groups. All LLLT doses also decreased (P<0.05) COX-2 compared to injury group at all time points, and to diclofenac group at 24h after trauma. In addition, LLLT decreased (P<0.05) TNF- compared both to injury and diclofenac groups at all time points. LLLT mainly with dose of 9J is better than topical application of diclofenac in acute inflammation after muscle trauma. (AU)

• Processo FAPESP: 10/52404-0 - Laserterapia de baixa potência na fadiga muscular e recuperação muscular pós-exercício: parâmetros ideais de aplicação e efeitos em exercício de longa duração
• Beneficiário: Ernesto Cesar Pinto Leal Junior
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores