Effects of cadmium and nitrogen on lipid composition of Chlorella vulgaris (Trebouxiophyceae, Chlorophyta)

Autor(es)

Chia, Mathias Ahii ^[1] ; Lombardi, Ana Teresa ^[1] ; Gama Melao, Maria Da Graca ^[2] ; Parrish, Christopher C. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Bot, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Hydrobiol, BR-13565905 Sao Carlos, SP - Brazil

^[3] Mem Univ Newfoundland, Ctr Ocean Sci, St John, NF A1C 5S7 - Canada

• Tipo de documento: Artigo Científico
• Fonte: European Journal of Phycology; v. 48, n. 1, p. 1-11, FEB 1 2013.
• Citações Web of Science: 27

Resumo

Investigations were carried out to evaluate lipid class and fatty acid composition of Chlorella vulgaris in relation to several combinations of cadmium (2x108; 107M Cd) and nitrogen (2.9x106 to 1.1x103 M N) concentrations. Triacylglycerols (TAG), acetone mobile polar lipids (AMPL) and phospholipids (PL) were the major lipid classes of C. vulgaris under all the tested conditions. TAG was the lipid class accumulated in the highest amount in N-limited C. vulgaris. The AMPL lipid class was lowest at the lowest N and highest Cd treatment. High proportions of saturated fatty acids (SAFA) and monounsaturated fatty acids (MUFA) in the total lipid extracts were recorded under N limitation in the presence of Cd. 16:1(n-11) was an exception among the MUFA as its levels decreased at low N. Total polyunsaturated FA (PUFA), 3 PUFA levels, and PUFA:SAFA ratios increased with increasing N concentrations, but when exposed to Cd, their levels were significantly reduced. Unlike the other PUFA, 18:2(n-6) increased with increasing N limitation. Significant negative relationships between TAG, MUFA and SAFA with N were recorded. Cd had a positive correlation with TAG, while N was with AMPL, PL and PUFA. In conclusion, a combination of N limitation and Cd stress significantly altered the lipid composition of C. vulgaris, and N limitation had the most significant overall effect on lipid class and fatty acid composition of the microalga. (AU)

• Processo FAPESP: 08/02078-9 - Estudos ecofisiológicos e de processos produtivos do Anostraca dulcícola Dendrocephalus brasiliensis Pesta 1921 e suas implicações nas comunidades zooplanctônica e nectônica
• Beneficiário: Maria da Graça Gama Melão
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/09694-0 - Ecofisiologia de células fitoplanctônicas cultivadas em fotobiorreator contínuo: estudos com Chlorella sorokiniana e Haematococcus pluvialis
• Beneficiário: Ana Teresa Lombardi
• Linha de fomento: Auxílio à Pesquisa - Regular