Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages

Autor(es)

Silva, Reinaldo C. ^[1] ; Landgraf, Maristella A. ^{[2, 3]} ; Correa-Costa, Matheus ^[3] ; Semedo, Patricia ^[1] ; Cenedeze, Marcos A. ^[1] ; Pacheco-Silva, Alvaro ^[1] ; Landgraf, Richardt G. ^[2] ; Camara, Niels O. S. ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Disciplina Nefrol, Lab Imunol Clin & Expt, BR-04023900 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Lab Inflamacao & Farmacol Vasc, BR-04023900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, Lab Imunobiol Transplante, BR-05508 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY; v. 31, n. 2-3, p. 179-188, 2013.
• Citações Web of Science: 8

Resumo

Background/Aims: Renal ischaemia-reperfusion injury (IRI) is a systemic inflammatory process in which Th1 responses predominate affecting other organs including the lungs. The present study explored the phagocytic and microbicidal capacity of macrophages in rats with lung inflammation that underwent IRI. Methods: The alveolar macrophages of rats sensitised to OVA were evaluated for phagocytosis and bacterial killing 24h after antigen challenge in animals with or without prior submission to 60 min of renal ischaemia. Results: Bronchoalveolar lavage had a high level of cellular infiltrate in immunised animals (420%) compared with control animals; IRI significantly reduced this infiltration (52%). Macrophages from animals immunised and challenged with OVA presented a 10x increase in phagocytic capacity compared to the control group, whereas immunised animals subjected to IRI showed a reduction in the phagocytic index of 68%. The killing of Klebsiella pneumoniae by macrophages from immunised animals was higher (56%) compared with the control group but reduced in animals submitted to IRI (45%). Immunised and challenged group showed an increase in gene expression levels of IL-10(450%), HO-1 (259%), INF-gamma (460%) and MCP-1 (370%) compared to the immunised group subjected to IRI. Conclusions: Renal ischaemia and reperfusion injury apparently alters the phagocytic and microbicidal capacity of macrophages, reducing lung inflammation to OVA. Copyright (C) 2013 S. Karger AG, Basel (AU)

• Processo FAPESP: 07/07139-3 - Investigando o papel da heme-oxigenase 1 em diferentes processos inflamatórios renais em modelos animais
• Beneficiário: Niels Olsen Saraiva Câmara
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/51104-8 - Mecanismos envolvidos na redução da resposta inflamatória pulmonar aguda e alérgica, em ratos Wistar desnutridos intra-uterinamente
• Beneficiário: Maristella de Almeida Vitta Landgraf
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 10/01404-0 - Estudos in vivo e in vitro da participação da leptina em diferentes modelos de inflamação pulmonar: mediadores inflamatórios e vias de sinalização envolvidas
• Beneficiário: Richardt Gama Landgraf
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 10/52180-4 - Fisiopatologia e regeneração das lesões renais
• Beneficiário: Nestor Schor
• Modalidade de apoio: Auxílio à Pesquisa - Temático